7162925
FcRH5, Mouse anti-Human, Clone: F25, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
FcRH5
Dilution
Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols
FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype
IgG2a κ
Purification Method
Protein G purified
Test Specificity
Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone
F25
Applications
ELISA, Flow Cytometry, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_112571.2
Immunogen
Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-FcRH5, clone F25, Cat
- No
- MABF2103, is a mouse monoclonal antibody that detects Fc receptor-like protein 5 and has been tested for use in ELISA, Flow Cytometry, and Western Blotting
- Fc receptor-like protein 5 (UniProt: Q96RD9; also known as FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, FcRH5, Immune receptor translocation-associated protein 2, IRTA2, CD307e) is encoded by the FCRL5 (also known as FCRH5, IRTA2, UNQ503/PRO820) gene (Gene ID: 83416) in human
- FcRH5 is a single-pass type I membrane protein that is expressed in marginal zone B-cells, immunoblasts, tonsillar germinal center centrocytes and in the intraepithelial and interfollicular regions of the tonsil
- It is involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages
- It is shown to regulate BCR signaling and binds aggregated IgG and engages several of its Ig-domains to bind IgG
- It is shown to be overexpressed in many lymphoma cell lines and on hairy cell leukemia cells
- Serum levels of soluble FcRH5 are reported to be elevated in patients with several types of B cell tumors
- FcRH5 is synthesized with a signal peptide (aa 1-15), which is cleaved off in the mature form that has a long extracellular domain (aa 16-851), a short transmembrane domain (aa 852-872), and a cytoplasmic tail (aa 873-977) that contains immunoreceptor tyrosine-based inhibitor motifs (ITIM)
- (Ref.: Franco, A., et al
- (2013)
- J
- Immunol
- 190(11); 5739-46; Ise, T., et al
- (2007)
- Leukemia 21(1); 169-174).
Compare Similar Items
Show Difference
Antigen: FcRH5
Dilution: Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols: FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone: F25
Applications: ELISA, Flow Cytometry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_112571.2
Immunogen: Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
FcRH5
Dilution:
Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols:
FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone:
F25
Applications:
ELISA, Flow Cytometry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_112571.2
Immunogen:
Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Chlamydial LPS
Dilution: Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Gene Symbols: __
Isotype: IgG2a
Purification Method: Protein G purified
Test Specificity: Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Clone: EVI-H1
Applications: Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial LPS
Dilution:
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Gene Symbols:
__
Isotype:
IgG2a
Purification Method:
Protein G purified
Test Specificity:
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Clone:
EVI-H1
Applications:
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Chlamydial HSP60
Dilution: Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia heat shock protein 60
Gene Symbols: N/A
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Clone: A57-B9
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial HSP60
Dilution:
Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia heat shock protein 60
Gene Symbols:
N/A
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Clone:
A57-B9
Applications:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD318 (CD6L)
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Gene Symbols: CDCP1;TRASK
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone: 3A11
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_073753.3
Immunogen: HBL-100 human mammary gland epithelial cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CD318 (CD6L)
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Gene Symbols:
CDCP1;TRASK
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone:
3A11
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_073753.3
Immunogen:
HBL-100 human mammary gland epithelial cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.