7162911
BMAL1 (ARNTL), Mouse anti-Mouse, Clone: B1BH2 , Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
BMAL1 (ARNTL)
Dilution
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Alias
Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Gene Symbols
ARNTL;BMAL1
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Clone
B1BH2
Applications
ChIP Assay, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Epigenetics & Nuclear Function
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001001463
Immunogen
MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-BMAL1 (ARNTL), clone B1BH2, Cat
- No
- MABE1886, is a highly specific mouse monoclonal antibody that targets Brain and muscle ARNT-like 1 (BMAL1) and has been tested for use in Chromatin Immunoprecipitation (ChIP) and Western Blotting
- Aryl hydrocarbon receptor nuclear translocator-like protein 1 (UniProt: Q9I8T7; also known as BMAL1b', Brain and muscle ARNT-like 1, cBMAL1) is encoded by the ARNTL (also known as BMAL1) gene (Gene ID: 374115) in chicken
- BMAL1 is expressed in pineal gland and retina and displays maximum levels between ZT13 and ZT16 during light/dark cycle
- BMAL1 is a transcriptional activator that forms a core component of the circadian clock
- These components include ARNTL/BMAL1, CLOCK, NPAS2, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1, and CRY2
- CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 form the positive limb of the feedback loop
- They act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes that are involved in key metabolic processes
- PER1/2/3 and CRY1/2, act as transcriptional repressors and form the negative limb of the feedback loop
- They interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity
- BMLA1 is highly expressed in the adult brain, skeletal muscle, and heart
- It shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription, and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer
- BMLA1 is reported to be phosphorylated upon dimerization with CLOCK and this phosphorylation enhances its transcriptional activity and reduces the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation
- BMAL1 null-mice are reported to have reproductive problems, reduced locomotor activity, and premature aging.
Compare Similar Items
Show Difference
Antigen: BMAL1 (ARNTL)
Dilution: Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Gene Symbols: ARNTL;BMAL1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Clone: B1BH2
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001001463
Immunogen: MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
BMAL1 (ARNTL)
Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Gene Symbols:
ARNTL;BMAL1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Clone:
B1BH2
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001001463
Immunogen:
MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Lap2alpha
Dilution: Western Blotting Analysis: A representative lot detected Lap2alpha in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lap2alpha in Chromatin Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunoprecipitation Analysis: A representative lot immunoprecipitated Lap2alpha in Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunofluorescence Analysis: A representative lot detected Lap2alpha in Immunofluorescence applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Lamina-associated polypeptide 2 isoform alpha;Thymopoietin isoforms alpha;TP alpha
Gene Symbols: Tmpo;Lap2
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 8C10-1H11 specifically detects Lamina-associated polypeptide 2 alpha (LAP2alpha) in murine species. It targets an epitope in the internal region.
Clone: 8C10-1H11
Applications: ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_035735
Immunogen: His-tagged recombinant fragment corresponding to 507 amino acids from the C-terminal region.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Lap2alpha
Dilution:
Western Blotting Analysis: A representative lot detected Lap2alpha in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lap2alpha in Chromatin Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunoprecipitation Analysis: A representative lot immunoprecipitated Lap2alpha in Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunofluorescence Analysis: A representative lot detected Lap2alpha in Immunofluorescence applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Lamina-associated polypeptide 2 isoform alpha;Thymopoietin isoforms alpha;TP alpha
Gene Symbols:
Tmpo;Lap2
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 8C10-1H11 specifically detects Lamina-associated polypeptide 2 alpha (LAP2alpha) in murine species. It targets an epitope in the internal region.
Clone:
8C10-1H11
Applications:
ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_035735
Immunogen:
His-tagged recombinant fragment corresponding to 507 amino acids from the C-terminal region.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD11a
Dilution: Flow Cytometry Analysis: A representative lot detected CD11a in Flow Cytometry applications (Baker, M.P., et. al. (1998). Blood. 92(8):2830-43).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Integrin alpha-L;CD11 antigen-like family member A;Leukocyte adhesion glycoprotein LFA-1 alpha chain;LFA-1A;Leukocyte function-associated molecule 1 alpha chain
Gene Symbols: ITGAL;CD11A
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone BU17 detects CD11a in human cells. It targets an epitope with in the extracellular domain.
Clone: BU17
Applications: Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_002200.2
Immunogen: Waldenstrom macroglobulinemia lymphoplasmacytic cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CD11a
Dilution:
Flow Cytometry Analysis: A representative lot detected CD11a in Flow Cytometry applications (Baker, M.P., et. al. (1998). Blood. 92(8):2830-43).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Integrin alpha-L;CD11 antigen-like family member A;Leukocyte adhesion glycoprotein LFA-1 alpha chain;LFA-1A;Leukocyte function-associated molecule 1 alpha chain
Gene Symbols:
ITGAL;CD11A
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone BU17 detects CD11a in human cells. It targets an epitope with in the extracellular domain.
Clone:
BU17
Applications:
Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_002200.2
Immunogen:
Waldenstrom macroglobulinemia lymphoplasmacytic cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CCR5 (CD195)
Dilution: Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195
Gene Symbols: CCR5;CMKBR5
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).
Clone: HEK/1/85a/7a
Applications: Flow Cytometry, Immunocytochemistry
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Inflammation & Immunology
Formulation: Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000570.1
Immunogen: CHO cells transfected with full length human CCR-5 (CD195).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CCR5 (CD195)
Dilution:
Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195
Gene Symbols:
CCR5;CMKBR5
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).
Clone:
HEK/1/85a/7a
Applications:
Flow Cytometry, Immunocytochemistry
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Inflammation & Immunology
Formulation:
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_000570.1
Immunogen:
CHO cells transfected with full length human CCR-5 (CD195).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.