7162915
CCR5 (CD195), Rat anti-Human, Clone: HEK/1/85a/7a, Millipore Sigma™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CCR5 (CD195)
Dilution
Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195
Gene Symbols
CCR5;CMKBR5
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).
Clone
HEK/1/85a/7a
Applications
Flow Cytometry, Immunocytochemistry
Conjugate
Unconjugated
Host Species
Rat
Research Discipline
Inflammation & Immunology
Formulation
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_000570.1
Immunogen
CHO cells transfected with full length human CCR-5 (CD195).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Description
- Anti-CCR5 (CD195), clone HEK/1/85a/7a, Cat
- No
- MABF2005, is a rat monoclonal antibody that detects C-C chemokine receptor type 5 (CCR5) and has been tested for use in Flow Cytometry and Immunocytochemistry
- C-C chemokine receptor type 5 (UniProt: P51681; also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195) is encoded by the CCR5 (also known as CMKBR5) gene (Gene ID: 1234) in human
- CCR-5 is a multi-pass membrane protein that is expressed in memory T-cells, macrophages, and immature dendritic cells, and is upregulated by proinflammatory cytokines
- It is also shown to be highly expressed in spleen, thymus, and on THP-1 and KG-1a cells
- Lower expression is observed in ovary and lung
- CCR-5 serves as a receptor for a number of inflammatory CC-chemokines and subsequently transduces a signal by increasing intracellular calcium levels
- It also plays a role in the control of granulocytic lineage proliferation or differentiation
- CCR-5 is acts as a co-receptor (CD4 being the primary receptor) of HIV-1 and HIV-2
- It also couples to Gi class of G-proteins to inhibit cAMP production and stimulate calcium release
- CCR-5 undergoes sulfation on two tyrosine residues in the N-terminal region, which increases efficiency of HIV-1 entry into cells
- It also undergoes phosphorylation at C-terminal serine residues following binding to CC chemokines, especially APO-RANTES
- Mutations in CCR5 gene have been linked to insulin-dependent diabetes mellitus
- (Ref.: Blanpain, C., et al
- (2002)
- Receptors Channels
- 8(1);19-31).
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Antigen: CCR5 (CD195)
Dilution: Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195
Gene Symbols: CCR5;CMKBR5
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).
Clone: HEK/1/85a/7a
Applications: Flow Cytometry, Immunocytochemistry
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Inflammation & Immunology
Formulation: Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000570.1
Immunogen: CHO cells transfected with full length human CCR-5 (CD195).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CCR5 (CD195)
Dilution:
Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195
Gene Symbols:
CCR5;CMKBR5
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).
Clone:
HEK/1/85a/7a
Applications:
Flow Cytometry, Immunocytochemistry
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Inflammation & Immunology
Formulation:
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_000570.1
Immunogen:
CHO cells transfected with full length human CCR-5 (CD195).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Siglec-H
Dilution: Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Sialic acid binding Ig-like lectin H;Siglech
Gene Symbols: Siglech;Siglec-H
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.
Clone: 551
Applications: Flow Cytometry, Immunohistochemistry
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Inflammation & Immunology
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_848821.2
Immunogen: Bone marrow-derived Type 1 interferon-producing cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Siglec-H
Dilution:
Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Sialic acid binding Ig-like lectin H;Siglech
Gene Symbols:
Siglech;Siglec-H
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.
Clone:
551
Applications:
Flow Cytometry, Immunohistochemistry
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Inflammation & Immunology
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_848821.2
Immunogen:
Bone marrow-derived Type 1 interferon-producing cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Siglec-H
Dilution: Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Sialic acid binding Ig-like lectin H;Siglech
Gene Symbols: Siglech;Siglec-H
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.
Clone: 551
Applications: Flow Cytometry, Immunohistochemistry
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Inflammation & Immunology
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_848821.2
Immunogen: Bone marrow-derived Type 1 interferon-producing cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Siglec-H
Dilution:
Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Sialic acid binding Ig-like lectin H;Siglech
Gene Symbols:
Siglech;Siglec-H
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.
Clone:
551
Applications:
Flow Cytometry, Immunohistochemistry
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Inflammation & Immunology
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_848821.2
Immunogen:
Bone marrow-derived Type 1 interferon-producing cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: NKp46 (CD335)
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Natural cytotoxicity triggering receptor 1;Lymphocyte antigen 94 homolog;NK cell-activating receptor;Natural killer cell p46-related protein;NK-p46;hNKp46;CD335
Gene Symbols: NCR1;LY94
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 461-G1 specifically recognizes whole human NKp46 and NKp46-D1, but does not recognize NKp46-D2. It targets an epitope with in the first 100 amino acids from the extracellular domain.
Clone: 461-G1
Applications: ELISA, Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001138929
Immunogen: Human IgG1 Fc-fused to a recombinant fragment corresponding to the first 100 amino acids from the extracellular domain of human NKp46.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
NKp46 (CD335)
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Natural cytotoxicity triggering receptor 1;Lymphocyte antigen 94 homolog;NK cell-activating receptor;Natural killer cell p46-related protein;NK-p46;hNKp46;CD335
Gene Symbols:
NCR1;LY94
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 461-G1 specifically recognizes whole human NKp46 and NKp46-D1, but does not recognize NKp46-D2. It targets an epitope with in the first 100 amino acids from the extracellular domain.
Clone:
461-G1
Applications:
ELISA, Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001138929
Immunogen:
Human IgG1 Fc-fused to a recombinant fragment corresponding to the first 100 amino acids from the extracellular domain of human NKp46.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.