7162917
Siglec-H, Rat anti-Mouse, Clone: 551, Millipore Sigma™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Siglec-H
Dilution
Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Alias
Sialic acid binding Ig-like lectin H;Siglech
Gene Symbols
Siglech;Siglec-H
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.
Clone
551
Applications
Flow Cytometry, Immunohistochemistry
Conjugate
Unconjugated
Host Species
Rat
Research Discipline
Inflammation & Immunology
Formulation
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_848821.2
Immunogen
Bone marrow-derived Type 1 interferon-producing cells.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Siglec-H, clone 551, Cat
- No
- MABF2045, is a rat monoclonal antibody that detects Sialic acid binding Ig-like lectin H and has been tested for use in Flow Cytometry and Immunohistochemistry
- Siglec-H (UniProt: Q3Y597; also known as Sialic acid binding Ig-like lectin H) is encoded by the Siglech gene (Gene ID: 233274) in murine species
- Siglecs are sialic acid-binding immunoglobulin-like lectins that are expressed mostly in the immune system and may be involved in both adhesive and signaling functions
- Siglec-H is a type I, CD33-related siglec-like transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form
- The mature form has to ig-like domains (aa 32-141 and 151-234) in its extracellular region
- Unlike other CD33-related siglecs, it lacks tyrosine-based signaling motifs in its cytoplasmic tail
- Siglec-H is shown to be expressed on plasmaocytoid dendritic cells (pDCs) and can serve as a reliable marker for these cells
- Siglec-H is also reported to serve as an endocytic receptor expressed on murine pDCs
- Action of Siglec-H depends on a 12 kDa DNAX-activating protein (DAP)
- (Ref.: Zhang, J., et al
- (2006)
- Blood 107(9); 3600-3608; Yun, TJ., et al., (2016), Cell Metabolism 23(5); 852-866).
Compare Similar Items
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Antigen: Siglec-H
Dilution: Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Sialic acid binding Ig-like lectin H;Siglech
Gene Symbols: Siglech;Siglec-H
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.
Clone: 551
Applications: Flow Cytometry, Immunohistochemistry
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Inflammation & Immunology
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_848821.2
Immunogen: Bone marrow-derived Type 1 interferon-producing cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Siglec-H
Dilution:
Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Sialic acid binding Ig-like lectin H;Siglech
Gene Symbols:
Siglech;Siglec-H
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.
Clone:
551
Applications:
Flow Cytometry, Immunohistochemistry
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Inflammation & Immunology
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_848821.2
Immunogen:
Bone marrow-derived Type 1 interferon-producing cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: NKp46 (CD335)
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Natural cytotoxicity triggering receptor 1;Lymphocyte antigen 94 homolog;NK cell-activating receptor;Natural killer cell p46-related protein;NK-p46;hNKp46;CD335
Gene Symbols: NCR1;LY94
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 461-G1 specifically recognizes whole human NKp46 and NKp46-D1, but does not recognize NKp46-D2. It targets an epitope with in the first 100 amino acids from the extracellular domain.
Clone: 461-G1
Applications: ELISA, Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001138929
Immunogen: Human IgG1 Fc-fused to a recombinant fragment corresponding to the first 100 amino acids from the extracellular domain of human NKp46.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
NKp46 (CD335)
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Natural cytotoxicity triggering receptor 1;Lymphocyte antigen 94 homolog;NK cell-activating receptor;Natural killer cell p46-related protein;NK-p46;hNKp46;CD335
Gene Symbols:
NCR1;LY94
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 461-G1 specifically recognizes whole human NKp46 and NKp46-D1, but does not recognize NKp46-D2. It targets an epitope with in the first 100 amino acids from the extracellular domain.
Clone:
461-G1
Applications:
ELISA, Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001138929
Immunogen:
Human IgG1 Fc-fused to a recombinant fragment corresponding to the first 100 amino acids from the extracellular domain of human NKp46.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD11b/CD18
Dilution: Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Non-human Primate
Gene Alias: Integrin alpha-M/ITGB2;CR3
Gene Symbols: ITGAM;CD11B;CR3A
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.
Clone: mAb107
Applications: Dot Blot, Flow Cytometry, Immunohistochemistry, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000623
Immunogen: GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD11b/CD18
Dilution:
Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Non-human Primate
Gene Alias:
Integrin alpha-M/ITGB2;CR3
Gene Symbols:
ITGAM;CD11B;CR3A
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.
Clone:
mAb107
Applications:
Dot Blot, Flow Cytometry, Immunohistochemistry, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_000623
Immunogen:
GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: SLAMF7 (CD319)
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
Gene Symbols: SLAMF7;CS1;UNQ576/PRO1138
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
Clone: 162
Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez): NP_067004.3
Immunogen: Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
SLAMF7 (CD319)
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
Gene Symbols:
SLAMF7;CS1;UNQ576/PRO1138
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
Clone:
162
Applications:
Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez):
NP_067004.3
Immunogen:
Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.