7162935
HLA-F, Mouse anti-Human, Clone: 3D11, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
HLA-F
Dilution
ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Gene Symbols
HLA-F;HLA-5.4;HLAF
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Clone
3D11
Applications
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_061823
Immunogen
Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-HLA-F, clone 3D11, Cat
- No
- MABF2162, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain F and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting
- HLA class I histocompatibility antigen, alpha chain F (UniProt: P30511; also known as CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F) is encoded by the HLA-F (also known as HLA-5.4, HLAF) gene (Gene ID: 3134) in human
- HLA-F is a single-pass type I membrane heterodimeric protein that is involved in the presentation of foreign antigens to the immune system
- It is expressed in B cells, NK cells, monocytes, and T cells and its expression is reported to be coincident with the activated immune response
- It is mainly localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types
- It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off
- The mature form has an extracellular domain (aa 22-305), a transmembrane domain (aa 306-329), and a short cytoplasmic domain (aa 330-346)
- The HLA-F locus has very low allelic polymorphism and is highly conserved in distantly related non-human primates indicating its conserved function
- It is involved in the regulation of immune system in pregnancy, infection, and autoimmunity by signaling through NK cell receptors
- (Ref.: Dulberger, CL et al
- (2017)
- Immunity 46(6); 1018 1029; Lee, N., et al
- (2010)
- Eur
- J
- Immunol
- 40(8): 2308 2318).
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Antigen: HLA-F
Dilution: ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Gene Symbols: HLA-F;HLA-5.4;HLAF
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Clone: 3D11
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_061823
Immunogen: Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
HLA-F
Dilution:
ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Gene Symbols:
HLA-F;HLA-5.4;HLAF
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Clone:
3D11
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_061823
Immunogen:
Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD33
Dilution: Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Gene Symbols: CD33;SIGLEC3
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone P67.6 specifically detects human CD 33.
Clone: P67.6
Applications: Flow Cytometry, Immunocytochemistry, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez): NP_001763.3
Immunogen: FMY9S5 cells expressing CD33.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD33
Dilution:
Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Gene Symbols:
CD33;SIGLEC3
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone P67.6 specifically detects human CD 33.
Clone:
P67.6
Applications:
Flow Cytometry, Immunocytochemistry, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez):
NP_001763.3
Immunogen:
FMY9S5 cells expressing CD33.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Chlamydial MOMP
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia major membrane protein
Gene Symbols: N/A
Isotype: IgG2a λ
Purification Method: Protein G purified
Test Specificity: Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
Clone: L21-10
Applications: Dot Blot, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Formalin killed Chlamydia trachomatis (L2/LGV-434).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial MOMP
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia major membrane protein
Gene Symbols:
N/A
Isotype:
IgG2a λ
Purification Method:
Protein G purified
Test Specificity:
Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
Clone:
L21-10
Applications:
Dot Blot, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Formalin killed Chlamydia trachomatis (L2/LGV-434).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD46
Dilution: Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen
Gene Symbols: CD46;MCP;MIC10
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone TRA-2-10 specifically detects CD46 in human cells.
Clone: TRA-2-10
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez): NP_002380
Immunogen: 2102Ep human embryonal carcinoma cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD46
Dilution:
Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen
Gene Symbols:
CD46;MCP;MIC10
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone TRA-2-10 specifically detects CD46 in human cells.
Clone:
TRA-2-10
Applications:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez):
NP_002380
Immunogen:
2102Ep human embryonal carcinoma cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.