7162939
CD46, Mouse anti-Human, Clone: TRA-2-10, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD46
Dilution
Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen
Gene Symbols
CD46;MCP;MIC10
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone TRA-2-10 specifically detects CD46 in human cells.
Clone
TRA-2-10
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez)
NP_002380
Immunogen
2102Ep human embryonal carcinoma cells.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Description
- Anti-CD46, clone TRA-2-10, Cat
- No
- MABF2182, is a mouse monoclonal antibody that detects Membrane cofactor protein (CD46) and has been for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Inhibition assay
- Membrane cofactor protein (UniProt: P15529; also known as TLX, Trophoblast leukocyte common antigen, CD46) is encoded by the CD46 (also known as MCP; MIC10) gene (Gene ID: 4179) in human
- CD46 is a single-pass type I membrane protein that is expressed by all cells except erythrocytes
- It acts as a cofactor for complement factor I, a serine protease that protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue
- It also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells
- CD46 is synthesized with a signal peptide (aa 1-34), which is cleaved off in the mature form
- The mature form contains an extracellular domain (aa 35-343), a transmembrane domain (aa 344-366), and a cytoplasmic domain (aa 367-392)
- It also contains four Sushi domains where Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles Virus H protein, domains 2 and 3 are essential for Herpes virus 6 binding, and domains 3 and 4 are required for interaction with Streptococcus Pyrogens M protein and are the most important for interaction with C3b and C4b
- CD46 can be glycosylated on Asn 83, 114, and 273 and N-glycosylation on Asn 114 and 273 are required for its cytoprotective function
- N-glycosylation on Asn 114 is also required for Measles virus binding and N-glycosylation on Asn-273 is required for Neisseria binding
- CD46 can also be extensively O-glycosylated in the Ser/Thr-rich domain, which is required for Neisseria binding, but not for Measles virus binding
- Mutations in CD46 gene have been linked to hemolytic uremic syndrome atypical 2 that has been characterized by microangiopathic hemolytic anemia, thrombocytopenia, and renal failure.
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Antigen: CD46
Dilution: Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen
Gene Symbols: CD46;MCP;MIC10
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone TRA-2-10 specifically detects CD46 in human cells.
Clone: TRA-2-10
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez): NP_002380
Immunogen: 2102Ep human embryonal carcinoma cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD46
Dilution:
Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen
Gene Symbols:
CD46;MCP;MIC10
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone TRA-2-10 specifically detects CD46 in human cells.
Clone:
TRA-2-10
Applications:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez):
NP_002380
Immunogen:
2102Ep human embryonal carcinoma cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: __
Dilution: __
Classification: __
Form: __
Regulatory Status: __
Target Species: __
Gene Alias: __
Gene Symbols: __
Isotype: __
Purification Method: __
Test Specificity: __
Clone: __
Applications: __
Conjugate: __
Host Species: __
Research Discipline: __
Formulation: __
Gene ID (Entrez): __
Immunogen: __
Primary or Secondary: __
Content And Storage: __
Antigen:
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Dilution:
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Classification:
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Form:
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Regulatory Status:
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Target Species:
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Gene Alias:
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Gene Symbols:
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Isotype:
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Purification Method:
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Test Specificity:
__
Clone:
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Applications:
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Conjugate:
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Host Species:
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Research Discipline:
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Formulation:
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Gene ID (Entrez):
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Immunogen:
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Primary or Secondary:
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Content And Storage:
__
Antigen: Neuritin (CPG15)
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat
Gene Alias: candidate plasticity gene 15;CPG15
Gene Symbols: NRN1;NRN
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone A6.1 detects human and rat Neuritin.
Clone: A6.1
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001265639
Immunogen: GST-tagged full-length human recombinant Neuritin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Neuritin (CPG15)
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Rat
Gene Alias:
candidate plasticity gene 15;CPG15
Gene Symbols:
NRN1;NRN
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone A6.1 detects human and rat Neuritin.
Clone:
A6.1
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001265639
Immunogen:
GST-tagged full-length human recombinant Neuritin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: phospho-Tau (Ser235)
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Alias: Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols: MAPT;MAPTL;MTBT1;TAU
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Clone: RN235
Applications: Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001116538
Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
phospho-Tau (Ser235)
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Alias:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols:
MAPT;MAPTL;MTBT1;TAU
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Clone:
RN235
Applications:
Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001116538
Immunogen:
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.