7162942
phospho-Tau (Ser235), Mouse anti-Human, Mouse, Clone: RN235, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
phospho-Tau (Ser235)
Dilution
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse
Gene Alias
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols
MAPT;MAPTL;MTBT1;TAU
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Clone
RN235
Applications
Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Neuroscience
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001116538
Immunogen
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Related Products
Description
- Anti-phospho-Tau (Ser235), clone RN235, Cat
- No
- MABN2275, is a highly specific mouse monoclonal antibody that targets Microtubule-associated protein tau and has been tested for use in Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting
- Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human
- Tau, a highly asymmetric and heat-stable protein, is expressed mainly in the brain, where it regulates the stability and orientation of microtubules in neurons, astrocytes, and oligodendrocytes
- Tau protein is reported to be the predominant component of the paired helical filaments (PHFs) and neurofibrillary tangles (NFTs) that are characteristic of pathological brain lesions in Alzheimer s disease
- The dephosphorylation of Tau protein promotes rapid and extensive microtubule polymerization, while the phosphorylation of Tau decreases its ability to promote microtubule assembly
- Tau is a substrate for a multitude of protein kinases, including CaM kinase II, casein kinase II, protein kinase A (PKA), MAP kinase (ERK2), Cdk5/ p35, and glycogen synthase kinase-3 (GSK-3) and is phosphorylated at many different sites in Alzheimer s disease brain
- It is believed that the phosphorylation of specific sites, rather than the overall extent of phosphorylation, is important in modulating the ability of Tau to bind microtubules and promote microtubule assembly
- This antibody (clone RN235) specifically reacts with tau phosphorylated at serine 235, and detects intraneuronal NFTs (iNFTs), and extraneuronal NFTs (eNFTs) in the brain tissue
- Although theoretically it can also react with phospho Thr231, but EC50 is very high compared to reactivity with phospho Ser235 (407 nM vs 0.61 nM)
- (Ref.: Brici, D., et al
- (2018)
- J
- Alzheimer s Dis
- 61(3); 899-905).
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Show Difference
Antigen: phospho-Tau (Ser235)
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Alias: Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols: MAPT;MAPTL;MTBT1;TAU
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Clone: RN235
Applications: Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001116538
Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
phospho-Tau (Ser235)
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Alias:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols:
MAPT;MAPTL;MTBT1;TAU
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Clone:
RN235
Applications:
Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001116538
Immunogen:
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Dab1
Dilution: Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Disabled homolog 1
Gene Symbols: Dab1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Clone: 4E12
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Neuroscience
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_034144.1
Immunogen: GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Dab1
Dilution:
Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Disabled homolog 1
Gene Symbols:
Dab1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Clone:
4E12
Applications:
Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Neuroscience
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_034144.1
Immunogen:
GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Dab1
Dilution: Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Disabled homolog 1
Gene Symbols: Dab1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Clone: 4E12
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Neuroscience
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_034144.1
Immunogen: GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Dab1
Dilution:
Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Disabled homolog 1
Gene Symbols:
Dab1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Clone:
4E12
Applications:
Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Neuroscience
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_034144.1
Immunogen:
GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Klotho
Dilution: Western Blotting Analysis: A represetative lot detected Klotho in Western Blotting applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunocytochemistry Analysis: A represetative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunoprecipitation Analysis: A represetative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).
Classification: Monoclonal
Form: __
Regulatory Status: RUO
Target Species: Human
Gene Alias: EC: 3.2.1.31;KL
Gene Symbols: KL
Isotype: IgG2a κ
Purification Method: Unpurified
Test Specificity: __
Clone: KL-115
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Neuroscience
Formulation: Rat monoclonal antibody in ascites with 0.1% sodium azide with 50% glycerol.
Gene ID (Entrez): NP_004786
Immunogen: GST-tagged recombinant fragment corresponding to 207 amino acids from the extracellular domain from the N-terminal region of human Klotho.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Klotho
Dilution:
Western Blotting Analysis: A represetative lot detected Klotho in Western Blotting applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunocytochemistry Analysis: A represetative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunoprecipitation Analysis: A represetative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
EC: 3.2.1.31;KL
Gene Symbols:
KL
Isotype:
IgG2a κ
Purification Method:
Unpurified
Test Specificity:
__
Clone:
KL-115
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Neuroscience
Formulation:
Rat monoclonal antibody in ascites with 0.1% sodium azide with 50% glycerol.
Gene ID (Entrez):
NP_004786
Immunogen:
GST-tagged recombinant fragment corresponding to 207 amino acids from the extracellular domain from the N-terminal region of human Klotho.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.