7162885
phospho AEP (Ser226), Rabbit anti-Human, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
phospho AEP (Ser226)
Applications
Immunofluorescence, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Neuroscience
Formulation
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez)
NP_005597
Immunogen
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen
∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification
Polyclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols
LGMN;PRSC1
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Related Products
Description
- Anti-phospho AEP (Ser226), Cat
- No
- ABN2270, is a highly specific rabbit polyclonal antibody that targets Asparaginyl endopeptidase (AEP) and has been tested in Immunofluorescence, serine-arginine protein kinase 2 (SRPK2) Assay, and Western Blotting
- Legumain (UniProt: Q99538; also known as EC: 3.4.22.34, Asparaginyl endopeptidase, AEP, Protease cysteine 1, Delta Secretase) is encoded by the LGMN (also known as PRSC1) gene (Gene ID: 5641) in human
- AEP is a lysosomal endopeptidase that displays strict specificity for the hydrolysis of asparaginyl bonds and can simultaneously cleave both amyloid precursor protein (APP) and tau
- It cleaves aspartyl bonds slowly, especially under acidic conditions
- AEP is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 324-433) that are cleaved off in the mature form
- It exists a homodimer before removal of the propeptide and after autocatalytic processing exists as a monomer
- In its zymogen form, the uncleaved propeptide blocks access to the active site
- AEP is phosphorylated on serine 226 by serine-arginine protein kinase 2 (SRPK2), which accelerates its autocatalytic cleavage and enhances its enzymatic activity
- The active form translocates to cytoplasm
- In Alzheimer s disease brains AEP is reported to be highly phosphorylated
- Depletion of AEP is shown to reduce amyloid peptide production and senile plaque formation in 5XFAD mouse brain that leads to restoration of synaptic activity and cognitive function
- Three isoforms of AEP have been described that are produced by alternative splicing
- (Ref.: Wang Z-H., et al
- (2017)
- Mol
- Cell 67(5); 812-825; Zhang, Z et al (2015)
- Nat
- Commun
- 6; p
- 9762).
Compare Similar Items
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Antigen: phospho AEP (Ser226)
Applications: Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez): NP_005597
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols: LGMN;PRSC1
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Antigen:
phospho AEP (Ser226)
Applications:
Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez):
NP_005597
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols:
LGMN;PRSC1
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Antigen: Aquaporin 4
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.
Gene ID (Entrez): NP_001257487.1
Immunogen: GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Rat, Mouse, Human
Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC
Gene Symbols: Aqp4
Isotype: __
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.
Antigen:
Aquaporin 4
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.
Gene ID (Entrez):
NP_001257487.1
Immunogen:
GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Rat, Mouse, Human
Gene Alias:
AQP-4;Mercurial-insensitive water channel;MIWC
Gene Symbols:
Aqp4
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.
Antigen: P2Y12 Receptor
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Signaling
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_073625
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the internal region of human P2Y purinoreceptor 12.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽50 kDa observed; 39.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250-1,000 dilution from a representative lot detected P2Y12 Receptor in human cerebral cortex and human tonsil tissue sections.Western Blotting Analysis: 1μg/mL from a representative lot detected P2Y12 Receptor in human platelet cell lysate.
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Rat, Human
Gene Alias: P2Y purinoceptor 12;ADP-glucose receptor;ADPG-R;P2T(AC);P2Y(AC);P2Y(cyc);P2Y12 platelet ADP receptor;P2Y(ADP);SP1999
Gene Symbols: P2RY12;HORK3
Isotype: __
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects P2Y12 receptor. It targets an epitope with in 13 amino acids from the extracellular domain.
Antigen:
P2Y12 Receptor
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Signaling
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_073625
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids from the internal region of human P2Y purinoreceptor 12.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽50 kDa observed; 39.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250-1,000 dilution from a representative lot detected P2Y12 Receptor in human cerebral cortex and human tonsil tissue sections.Western Blotting Analysis: 1μg/mL from a representative lot detected P2Y12 Receptor in human platelet cell lysate.
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Rat, Human
Gene Alias:
P2Y purinoceptor 12;ADP-glucose receptor;ADPG-R;P2T(AC);P2Y(AC);P2Y(cyc);P2Y12 platelet ADP receptor;P2Y(ADP);SP1999
Gene Symbols:
P2RY12;HORK3
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects P2Y12 receptor. It targets an epitope with in 13 amino acids from the extracellular domain.
Antigen: Frizzled-2
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Signaling
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal half of human Frizzled-2.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽60 kDa observed; 63.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: __
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Human
Gene Alias: Fz-2;hFz2;FzE2
Gene Symbols: FZD2
Isotype: __
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects human Frizzled -2. It targets an epitope with in 13 amino acids from the extracellular domain.
Antigen:
Frizzled-2
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Signaling
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal half of human Frizzled-2.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽60 kDa observed; 63.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
__
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Fz-2;hFz2;FzE2
Gene Symbols:
FZD2
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects human Frizzled -2. It targets an epitope with in 13 amino acids from the extracellular domain.