7162943
Dab1, Rat anti-Mouse, Clone: 4E12, Millipore Sigma™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Dab1
Dilution
Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Alias
Disabled homolog 1
Gene Symbols
Dab1
Isotype
IgG2a κ
Purification Method
Protein G purified
Test Specificity
Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Clone
4E12
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Rat
Research Discipline
Neuroscience
Formulation
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_034144.1
Immunogen
GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Dab1, clone 4E12, Cat
- No
- MABN2276, is a highly specific rat monoclonal antibody that targets Disabled homolog 1 and has been tested in Immunohistochemistry, Immunoprecipitation, and Western Blotting
- Disabled homolog 1 (UniProt: P97318; also known as DAB1) is encoded by the Dab1 gene (Gene ID: 13131) in murine species
- Dab 1 is mainly expressed in the brain and serves as an adapter molecule involved in neural development where it is reported to direct the migration of cortical neurons past previously formed neurons to reach their proper layer
- It is a cytosolic protein that is shown to be essential component of the reelin signaling pathway
- It is shown to bind to the cytoplasmic tail of Reelin receptor, APO E receptor 2, and to LDL receptor
- Dab1 can be phosphorylated on Tyr198, Tyr220, and Tyr232 upon reelin induction in embryonic neurons
- It can also be phosphorylated on Ser524, but this phosphorylation is independent of reelin signaling
- Tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is considered to be the most critical downstream event in Reelin signaling
- Dab1 contains a phosphotyrosine interaction domain (PID) (aa 36-189), which specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins
- Seven different isoforms of Dab1 have been identified that are generated via alternative splicing
- Clone 4E12 binds to an epitope within amino acids 161-320
- (Ref.: Onoue, A., et al
- (2014)
- Neurosci
- Res
- 88; 23-27).
Compare Similar Items
Show Difference
Antigen: Dab1
Dilution: Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Disabled homolog 1
Gene Symbols: Dab1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Clone: 4E12
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Neuroscience
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_034144.1
Immunogen: GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Dab1
Dilution:
Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Disabled homolog 1
Gene Symbols:
Dab1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Clone:
4E12
Applications:
Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Neuroscience
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_034144.1
Immunogen:
GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Klotho
Dilution: Western Blotting Analysis: A represetative lot detected Klotho in Western Blotting applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunocytochemistry Analysis: A represetative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunoprecipitation Analysis: A represetative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).
Classification: Monoclonal
Form: __
Regulatory Status: RUO
Target Species: Human
Gene Alias: EC: 3.2.1.31;KL
Gene Symbols: KL
Isotype: IgG2a κ
Purification Method: Unpurified
Test Specificity: __
Clone: KL-115
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Neuroscience
Formulation: Rat monoclonal antibody in ascites with 0.1% sodium azide with 50% glycerol.
Gene ID (Entrez): NP_004786
Immunogen: GST-tagged recombinant fragment corresponding to 207 amino acids from the extracellular domain from the N-terminal region of human Klotho.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Klotho
Dilution:
Western Blotting Analysis: A represetative lot detected Klotho in Western Blotting applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunocytochemistry Analysis: A represetative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunoprecipitation Analysis: A represetative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
EC: 3.2.1.31;KL
Gene Symbols:
KL
Isotype:
IgG2a κ
Purification Method:
Unpurified
Test Specificity:
__
Clone:
KL-115
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Neuroscience
Formulation:
Rat monoclonal antibody in ascites with 0.1% sodium azide with 50% glycerol.
Gene ID (Entrez):
NP_004786
Immunogen:
GST-tagged recombinant fragment corresponding to 207 amino acids from the extracellular domain from the N-terminal region of human Klotho.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: HAP1
Dilution: Immunocytochemistry Analysis: A representative lot detected HAP1 in Immunocytochemistry applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Immunoprecipitation Analysis: A representative lot immunoprecipitated HAP1 in Immunoprecipitation applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Western Blotting Analysis: A representative lot detected HAP1 in Western Blotting applications (Chan, E.Y., et. al (2002). Hum Mol Genet. 11(8):945-59; Tang, T.S., et. al. (2003). Neuron. 39(2):227-39; Torre, E.R., et. al. (2003). J Neurosci Methods. 125(1-2):27-32).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Rat
Gene Alias: Huntingtin-associated protein 1
Gene Symbols: Hap1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 1B6 detects both HAP1A and HAP1B proteins in mouse and rat brain. It targets an epitope from the internal region.
Clone: 1B6
Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_077047.1
Immunogen: GST-tagged recombinant fragment corresponding to 137 amino acids from the internal region of rat Huntingtin-associated protein 1 (HAP1).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
HAP1
Dilution:
Immunocytochemistry Analysis: A representative lot detected HAP1 in Immunocytochemistry applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Immunoprecipitation Analysis: A representative lot immunoprecipitated HAP1 in Immunoprecipitation applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Western Blotting Analysis: A representative lot detected HAP1 in Western Blotting applications (Chan, E.Y., et. al (2002). Hum Mol Genet. 11(8):945-59; Tang, T.S., et. al. (2003). Neuron. 39(2):227-39; Torre, E.R., et. al. (2003). J Neurosci Methods. 125(1-2):27-32).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Rat
Gene Alias:
Huntingtin-associated protein 1
Gene Symbols:
Hap1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 1B6 detects both HAP1A and HAP1B proteins in mouse and rat brain. It targets an epitope from the internal region.
Clone:
1B6
Applications:
Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_077047.1
Immunogen:
GST-tagged recombinant fragment corresponding to 137 amino acids from the internal region of rat Huntingtin-associated protein 1 (HAP1).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Aquaporin-4
Dilution: Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat, Mouse
Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols: AQP4
Isotype: IgG3 κ
Purification Method: Protein G purified
Test Specificity: Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.
Clone: mCCD
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG3 in PBS without preservatives.
Gene ID (Entrez): NP_001641
Immunogen: His-tagged full length human recombinant Aquaporin-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Aquaporin-4
Dilution:
Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Rat, Mouse
Gene Alias:
AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols:
AQP4
Isotype:
IgG3 κ
Purification Method:
Protein G purified
Test Specificity:
Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.
Clone:
mCCD
Applications:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG3 in PBS without preservatives.
Gene ID (Entrez):
NP_001641
Immunogen:
His-tagged full length human recombinant Aquaporin-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.