7162946
HAP1, Mouse anti-Mouse, Rat, Clone: 1B6, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
HAP1
Dilution
Immunocytochemistry Analysis: A representative lot detected HAP1 in Immunocytochemistry applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Immunoprecipitation Analysis: A representative lot immunoprecipitated HAP1 in Immunoprecipitation applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Western Blotting Analysis: A representative lot detected HAP1 in Western Blotting applications (Chan, E.Y., et. al (2002). Hum Mol Genet. 11(8):945-59; Tang, T.S., et. al. (2003). Neuron. 39(2):227-39; Torre, E.R., et. al. (2003). J Neurosci Methods. 125(1-2):27-32).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse, Rat
Gene Alias
Huntingtin-associated protein 1
Gene Symbols
Hap1
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 1B6 detects both HAP1A and HAP1B proteins in mouse and rat brain. It targets an epitope from the internal region.
Clone
1B6
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Neuroscience
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_077047.1
Immunogen
GST-tagged recombinant fragment corresponding to 137 amino acids from the internal region of rat Huntingtin-associated protein 1 (HAP1).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-HAP1, clone 1B6, Cat
- No
- MABN2456, is a highly specific mouse monoclonal antibody that targets Huntingtin-associated protein 1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting
- Huntingtin-associated protein 1 (UniProt: P54256; also known as HAP1) is encoded by the Hap1 gene (Gene ID: 29430) in rat
- HAP1 is a huntingtin-associated protein that is enriched in the brain
- It specifically associates with HTT/huntingtin and this binding is enhanced by an expanded polyglutamine repeats within HTT that affects HAP1 interaction properties
- Both HTT and HAP1 are reported to be involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos
- HAP1 localizes to large nonmembrane-bound cytoplasmic bodies (stigmoid bodies) in various types of neurons
- The formation of stigmoid bodies is regulated by the ratio of isoform A to isoform B of HAP1
- HAP1 plays a role in vesicular transport within neurons that involves microtubule-associated transporters and is shown to be attenuated by association with mutant HTT
- In rodents, two HAP1 isoforms (HAP1A and HAP1B) have been described that differ in their carboxy terminal
- However, humans contain only the HAP1A isoform
- In murine models, targeted disruption of the HAP1 gene results in significantly depressed feeding behavior and premature postnatal death
- Pups with Hap1 -/- show decreased feeding, malnutrition, dehydration, and premature death
- (Ref.: Tang, TS et al., (2003)
- Neuron 39 (2); 227-239; Chan, EY., et al
- (2002)
- Hum
- Mol
- Gen
- 11(8); 945-959).
Compare Similar Items
Show Difference
Antigen: HAP1
Dilution: Immunocytochemistry Analysis: A representative lot detected HAP1 in Immunocytochemistry applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Immunoprecipitation Analysis: A representative lot immunoprecipitated HAP1 in Immunoprecipitation applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Western Blotting Analysis: A representative lot detected HAP1 in Western Blotting applications (Chan, E.Y., et. al (2002). Hum Mol Genet. 11(8):945-59; Tang, T.S., et. al. (2003). Neuron. 39(2):227-39; Torre, E.R., et. al. (2003). J Neurosci Methods. 125(1-2):27-32).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Rat
Gene Alias: Huntingtin-associated protein 1
Gene Symbols: Hap1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 1B6 detects both HAP1A and HAP1B proteins in mouse and rat brain. It targets an epitope from the internal region.
Clone: 1B6
Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_077047.1
Immunogen: GST-tagged recombinant fragment corresponding to 137 amino acids from the internal region of rat Huntingtin-associated protein 1 (HAP1).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
HAP1
Dilution:
Immunocytochemistry Analysis: A representative lot detected HAP1 in Immunocytochemistry applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Immunoprecipitation Analysis: A representative lot immunoprecipitated HAP1 in Immunoprecipitation applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).Western Blotting Analysis: A representative lot detected HAP1 in Western Blotting applications (Chan, E.Y., et. al (2002). Hum Mol Genet. 11(8):945-59; Tang, T.S., et. al. (2003). Neuron. 39(2):227-39; Torre, E.R., et. al. (2003). J Neurosci Methods. 125(1-2):27-32).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Rat
Gene Alias:
Huntingtin-associated protein 1
Gene Symbols:
Hap1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 1B6 detects both HAP1A and HAP1B proteins in mouse and rat brain. It targets an epitope from the internal region.
Clone:
1B6
Applications:
Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_077047.1
Immunogen:
GST-tagged recombinant fragment corresponding to 137 amino acids from the internal region of rat Huntingtin-associated protein 1 (HAP1).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Aquaporin-4
Dilution: Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat, Mouse
Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols: AQP4
Isotype: IgG3 κ
Purification Method: Protein G purified
Test Specificity: Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.
Clone: mCCD
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG3 in PBS without preservatives.
Gene ID (Entrez): NP_001641
Immunogen: His-tagged full length human recombinant Aquaporin-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Aquaporin-4
Dilution:
Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Rat, Mouse
Gene Alias:
AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols:
AQP4
Isotype:
IgG3 κ
Purification Method:
Protein G purified
Test Specificity:
Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.
Clone:
mCCD
Applications:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG3 in PBS without preservatives.
Gene ID (Entrez):
NP_001641
Immunogen:
His-tagged full length human recombinant Aquaporin-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Aquaporin-4
Dilution: Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat, Mouse
Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols: AQP4
Isotype: IgG3 κ
Purification Method: Protein G purified
Test Specificity: Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.
Clone: mCCD
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG3 in PBS without preservatives.
Gene ID (Entrez): NP_001641
Immunogen: His-tagged full length human recombinant Aquaporin-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Aquaporin-4
Dilution:
Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Rat, Mouse
Gene Alias:
AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols:
AQP4
Isotype:
IgG3 κ
Purification Method:
Protein G purified
Test Specificity:
Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.
Clone:
mCCD
Applications:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG3 in PBS without preservatives.
Gene ID (Entrez):
NP_001641
Immunogen:
His-tagged full length human recombinant Aquaporin-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Aquaporin-4
Dilution: Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat, Human, Mouse
Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols: AQP4
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.
Clone: mECD
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez): NP_001641
Immunogen: His-tagged full-length human recombinant Aquaporin-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Aquaporin-4
Dilution:
Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat, Human, Mouse
Gene Alias:
AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols:
AQP4
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.
Clone:
mECD
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez):
NP_001641
Immunogen:
His-tagged full-length human recombinant Aquaporin-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.