7162948
Aquaporin-4, Mouse anti-Rat, Human, Mouse, Clone: mECD, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Aquaporin-4
Dilution
Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Rat, Human, Mouse
Gene Alias
AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols
AQP4
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.
Clone
mECD
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Neuroscience
Formulation
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez)
NP_001641
Immunogen
His-tagged full-length human recombinant Aquaporin-4.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Description
- Anti-Aquaporin-4, clone mECD, Cat
- No
- MABN2471, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation
- Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human
- Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system
- Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323)
- Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue
- Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%
- Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing
- Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NMO) from multiple sclerosis
- Clone mECD is shown to induce clustering and internalization of Aquporin-4 and cause its redistribution, which is also observed with NMO-IgG
- Exposure to mECD antibody is also shown to cause coaggregation of astrocytic CD28B and Aquaporin-4
- (Ref.: Hinson, SR et al
- (2017)
- Proc
- Natl.Acad
- Sci
- USA 114(21); 5491-5496).
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Antigen: Aquaporin-4
Dilution: Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat, Human, Mouse
Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols: AQP4
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.
Clone: mECD
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez): NP_001641
Immunogen: His-tagged full-length human recombinant Aquaporin-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Aquaporin-4
Dilution:
Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat, Human, Mouse
Gene Alias:
AQP-4;Mercurial-insensitive water channel;MIWC;WCH4
Gene Symbols:
AQP4
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.
Clone:
mECD
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez):
NP_001641
Immunogen:
His-tagged full-length human recombinant Aquaporin-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: NMDAR2B
Dilution: Western Blotting Analysis: A representative lot detected NMDAR2B in Western Blotting applications (Lu, W., et. al. (2017). Science. 355(6331)). Western Blotting Analysis: 4μg/mL from a representative lot detected NMDAR2B in mouse brain tissue lysate.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat
Gene Alias: Glutamate receptor ionotropic NMDA 2B;GluN2B;Glutamate [NMDA] receptor subunit epsilon-2;N-methyl D-aspartate receptor subtype 2B;NR2B;N-methyl-D-aspartate receptor subunit 3;NR3;hNR3
Gene Symbols: GRIN2B;NMDAR2B
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 11D1 is a mouse monoclonal antibody that specifically detects NMDAR2B.
Clone: 11D1
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000825
Immunogen: Full length purified human Glutamate receptor ionotropic, NMDA 2B (NMDAR2B).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
NMDAR2B
Dilution:
Western Blotting Analysis: A representative lot detected NMDAR2B in Western Blotting applications (Lu, W., et. al. (2017). Science. 355(6331)). Western Blotting Analysis: 4μg/mL from a representative lot detected NMDAR2B in mouse brain tissue lysate.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat
Gene Alias:
Glutamate receptor ionotropic NMDA 2B;GluN2B;Glutamate [NMDA] receptor subunit epsilon-2;N-methyl D-aspartate receptor subtype 2B;NR2B;N-methyl-D-aspartate receptor subunit 3;NR3;hNR3
Gene Symbols:
GRIN2B;NMDAR2B
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 11D1 is a mouse monoclonal antibody that specifically detects NMDAR2B.
Clone:
11D1
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_000825
Immunogen:
Full length purified human Glutamate receptor ionotropic, NMDA 2B (NMDAR2B).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ErbB4
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected ErbB4 in mouse embryo tissue sections.Western Blotting Analysis: A representative lot detected ErbB4 in Western Blotting applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).ELISA Analysis: A representative lot detected ErbB4 in ELISA applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).Immunohistochemistry Analysis: A representative lot detected ErbB4 in Immunohistochemistry applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).Immunoprecipitation Analysis: A representative lot immunoprecipitated ErbB4 in Immunoprecipitation applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Receptor tyrosine-protein kinase erbB-4;Proto-oncogene-like protein c-ErbB-4
Gene Symbols: Erbb4;Mer4
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: Clone mAB10 is a rabbit monoclonal antibody that detects mouse Receptor tyrosine-protein kinase erbB-4. It targets an epitope with in the C-terminal region.
Clone: mab10
Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_034284.1
Immunogen: GST-tagged recombinant protein corresponding to 204 amino acids from the C-terminal region of murine Receptor tyrosine-protein kinase erbB-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ErbB4
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected ErbB4 in mouse embryo tissue sections.Western Blotting Analysis: A representative lot detected ErbB4 in Western Blotting applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).ELISA Analysis: A representative lot detected ErbB4 in ELISA applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).Immunohistochemistry Analysis: A representative lot detected ErbB4 in Immunohistochemistry applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).Immunoprecipitation Analysis: A representative lot immunoprecipitated ErbB4 in Immunoprecipitation applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Receptor tyrosine-protein kinase erbB-4;Proto-oncogene-like protein c-ErbB-4
Gene Symbols:
Erbb4;Mer4
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
Clone mAB10 is a rabbit monoclonal antibody that detects mouse Receptor tyrosine-protein kinase erbB-4. It targets an epitope with in the C-terminal region.
Clone:
mab10
Applications:
ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_034284.1
Immunogen:
GST-tagged recombinant protein corresponding to 204 amino acids from the C-terminal region of murine Receptor tyrosine-protein kinase erbB-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Cdc42
Dilution: Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Human, Yeast
Gene Alias: Cell division control protein 42;Suppressor of RHO3 protein 2
Gene Symbols: CDC42;SRO2;TLR229C;L8083.13
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 28-10 specifically detects Cell division control protein 42 (Cdc42).
Clone: 28-10
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_013330
Immunogen: GST-tagged full-length recombinant yeast Cdc42.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Cdc42
Dilution:
Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Human, Yeast
Gene Alias:
Cell division control protein 42;Suppressor of RHO3 protein 2
Gene Symbols:
CDC42;SRO2;TLR229C;L8083.13
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 28-10 specifically detects Cell division control protein 42 (Cdc42).
Clone:
28-10
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_013330
Immunogen:
GST-tagged full-length recombinant yeast Cdc42.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.