7162951
Cdc42, Mouse anti-Mouse, Yeast, Human, Clone: 28-10, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Cdc42
Dilution
Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse, Human, Yeast
Gene Alias
Cell division control protein 42;Suppressor of RHO3 protein 2
Gene Symbols
CDC42;SRO2;TLR229C;L8083.13
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 28-10 specifically detects Cell division control protein 42 (Cdc42).
Clone
28-10
Applications
Immunocytochemistry, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Neuroscience
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_013330
Immunogen
GST-tagged full-length recombinant yeast Cdc42.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Cdc42, clone 28-10, Cat
- No
- MABN2485, is a highly specific mouse monoclonal antibody that targets Cell division control protein 42 (Cdc42) and has been tested for use in Immunocytochemistry and Western Blotting
- Cell division control protein 42 (UniProt: P19073; also known as Cdc42, Suppressor of RHO3 protein 2) is encoded by the CDC42 (also known as SRO2, TLR229C, L8083.13) gene (Gene ID: 850930) in yeast (Saccharomyces cerevisiae)
- Cdc42 is a member of the Rho GTPase family that regulates multiple signaling pathways involved in actomyosin organization, cell proliferation, and maintenance of cell polarity in most eukaryotic cell types
- Cdc42 signaling process involves guanine nucleotide exchange factors (GEFs), guanine nucleotide dissociation inhibitors (GDIs), and GTPase-activating proteins (GAPs)
- In yeast, generation and maintenance of robust Cdc42 polarity promotes membrane expansion during bud formation and it has been shown that Cdc42 is dynamically maintained at the polarity cap through its continuous cycling between the polarity cap and internal pools
- Cdc42 is highly polarized during bud emergence and is specifically required for exocytosis at this stage
- Cdc42 is synthesized with a propeptide region (aa 189-191), which is cleaved off in the mature form
- The mature form contains three nucleotide (GTP)-binding sites
- (Ref.: Wu, H., and Brennwald, P
- (2010) Mol
- Cell Biol
- 30(21); 5207-5217).
Compare Similar Items
Show Difference
Antigen: Cdc42
Dilution: Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Human, Yeast
Gene Alias: Cell division control protein 42;Suppressor of RHO3 protein 2
Gene Symbols: CDC42;SRO2;TLR229C;L8083.13
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 28-10 specifically detects Cell division control protein 42 (Cdc42).
Clone: 28-10
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_013330
Immunogen: GST-tagged full-length recombinant yeast Cdc42.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Cdc42
Dilution:
Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Human, Yeast
Gene Alias:
Cell division control protein 42;Suppressor of RHO3 protein 2
Gene Symbols:
CDC42;SRO2;TLR229C;L8083.13
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 28-10 specifically detects Cell division control protein 42 (Cdc42).
Clone:
28-10
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_013330
Immunogen:
GST-tagged full-length recombinant yeast Cdc42.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: VMAT1
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat, Mouse
Gene Alias: Chromaffin granule amine transporter;Solute carrier family 18 member 1;Vesicular amine transporter 1;VAT1
Gene Symbols: Scl18a1;Vmat1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.
Clone: N440/21
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Gene ID (Entrez): NP_694694.1
Immunogen: Recombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
VMAT1
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat, Mouse
Gene Alias:
Chromaffin granule amine transporter;Solute carrier family 18 member 1;Vesicular amine transporter 1;VAT1
Gene Symbols:
Scl18a1;Vmat1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.
Clone:
N440/21
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Gene ID (Entrez):
NP_694694.1
Immunogen:
Recombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: VMAT1
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat, Mouse
Gene Alias: Chromaffin granule amine transporter;Solute carrier family 18 member 1;Vesicular amine transporter 1;VAT1
Gene Symbols: Scl18a1;Vmat1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.
Clone: N440/21
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Gene ID (Entrez): NP_694694.1
Immunogen: Recombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
VMAT1
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat, Mouse
Gene Alias:
Chromaffin granule amine transporter;Solute carrier family 18 member 1;Vesicular amine transporter 1;VAT1
Gene Symbols:
Scl18a1;Vmat1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.
Clone:
N440/21
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Gene ID (Entrez):
NP_694694.1
Immunogen:
Recombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: MULT1
Dilution: Immunocytochemistry Analysis: A representative lot detected MULT1 in Immunocytochemistry applications (Lenac, T., et. al. (2006). J Exp Med. 203(8):1843-50; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).Flow Cytometry Analysis: A representative lot detected MULT1 in Flow Cytometry applications (Lenac, T., et. al. (2006). J Exp Med. 203(8):1843-50; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).ELISA Analysis: A representative lot detected MULT1 in ELISA applications (Sheppard, S., et. al. (2017). Nat Commun. 8:13930; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: MULT-1;ULBP1;mouse UL16-binding protein-like Transcript 1;NKG2DL;NKG2D ligand
Gene Symbols: Ulbp1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 1D6 is a rat monoclonal antibody that detects UL16-binding protein-like transcript 1 splicing variant in mouse.
Clone: 1D6
Applications: ELISA, Flow Cytometry, Immunocytochemistry
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Signaling
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NA
Immunogen: Recombinant fragment corresponding to 186 amino acids from the extracellular domain of murine UL16-binding protein-like transcript 1 splicing variant (MULT-1).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
MULT1
Dilution:
Immunocytochemistry Analysis: A representative lot detected MULT1 in Immunocytochemistry applications (Lenac, T., et. al. (2006). J Exp Med. 203(8):1843-50; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).Flow Cytometry Analysis: A representative lot detected MULT1 in Flow Cytometry applications (Lenac, T., et. al. (2006). J Exp Med. 203(8):1843-50; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).ELISA Analysis: A representative lot detected MULT1 in ELISA applications (Sheppard, S., et. al. (2017). Nat Commun. 8:13930; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
MULT-1;ULBP1;mouse UL16-binding protein-like Transcript 1;NKG2DL;NKG2D ligand
Gene Symbols:
Ulbp1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 1D6 is a rat monoclonal antibody that detects UL16-binding protein-like transcript 1 splicing variant in mouse.
Clone:
1D6
Applications:
ELISA, Flow Cytometry, Immunocytochemistry
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Signaling
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NA
Immunogen:
Recombinant fragment corresponding to 186 amino acids from the extracellular domain of murine UL16-binding protein-like transcript 1 splicing variant (MULT-1).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.