7162936
CD33, Mouse anti-Human, Clone: P67.6, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD33
Dilution
Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Gene Symbols
CD33;SIGLEC3
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone P67.6 specifically detects human CD 33.
Clone
P67.6
Applications
Flow Cytometry, Immunocytochemistry, Inhibition Assays
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez)
NP_001763.3
Immunogen
FMY9S5 cells expressing CD33.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Description
- Anti-CD33, clone P67.6, Cat
- No
- MABF2163, is a mouse monoclonal antibody that detects CD33 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition assays
- Myeloid cell surface antigen CD33 (UniProt: P20138; also known as Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33) is encoded by the CD33 (also known as SIGLEC3) gene (Gene ID: 945) in human
- CD33 is a single-pass type I membrane sialoadhesion protein that is expressed by myeloid stem cells, myeloblasts, monoblasts, monocytes, macrophages, and granulocyte precursors and preferentially binds to alpha-2,6-linked sialic acid
- CD33 is not expressed in erythrocytes, platelets, B-cells, T-cells, and NK cells
- Hence, it can serve as a marker for myeloid cells
- It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form
- It contains an extracellular domain (aa 18-259), a transmembrane domain (aa 260-282), and a cytoplasmic domain (aa 283-364)
- CD33 contains two copies of a cytoplasmic motif (aa 338-343 and 356-361), which is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM)
- This motif is involved in modulation of cellular responses
- In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules
- Human CD33 is reported to contain two tyrosine residues in its cytoplasmic domain (tyrosine 340 and 358) that when phosphorylated could function as docking sites for the phosphatases, SHP-1 and/or SHP-2, and enable CD33 to function as an inhibitory receptor.
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Antigen: CD33
Dilution: Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Gene Symbols: CD33;SIGLEC3
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone P67.6 specifically detects human CD 33.
Clone: P67.6
Applications: Flow Cytometry, Immunocytochemistry, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez): NP_001763.3
Immunogen: FMY9S5 cells expressing CD33.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD33
Dilution:
Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Gene Symbols:
CD33;SIGLEC3
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone P67.6 specifically detects human CD 33.
Clone:
P67.6
Applications:
Flow Cytometry, Immunocytochemistry, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez):
NP_001763.3
Immunogen:
FMY9S5 cells expressing CD33.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Chlamydial MOMP
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia major membrane protein
Gene Symbols: N/A
Isotype: IgG2a λ
Purification Method: Protein G purified
Test Specificity: Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
Clone: L21-10
Applications: Dot Blot, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Formalin killed Chlamydia trachomatis (L2/LGV-434).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial MOMP
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia major membrane protein
Gene Symbols:
N/A
Isotype:
IgG2a λ
Purification Method:
Protein G purified
Test Specificity:
Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
Clone:
L21-10
Applications:
Dot Blot, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Formalin killed Chlamydia trachomatis (L2/LGV-434).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD46
Dilution: Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen
Gene Symbols: CD46;MCP;MIC10
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone TRA-2-10 specifically detects CD46 in human cells.
Clone: TRA-2-10
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez): NP_002380
Immunogen: 2102Ep human embryonal carcinoma cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD46
Dilution:
Inhibition Analysis: A representative lot blocked Measles virus infection in BC1 cells. (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7). Immunohistochemistry Analysis: A representative lot detected CD46 in Immunohistochemistry applications (Kemper, C., et. al. (2001). Clin Exp Immunol. 124(2):180-9).Immunoprecipitation Analysis: A representative lot immunoprecipitated CD46 in Immunoprecipitation applications (Andrews, P.W., et. al. (1985). Ann Hum Genet. 49(Pt 1):31-9).Flow Cytometry Analysis: A representative lot detected CD46 in Flow Cytometry applications (Manchester, M., et. al. (1995). Proc Natl Acad Sci U S A. 92(6):2303-7).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen
Gene Symbols:
CD46;MCP;MIC10
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone TRA-2-10 specifically detects CD46 in human cells.
Clone:
TRA-2-10
Applications:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Gene ID (Entrez):
NP_002380
Immunogen:
2102Ep human embryonal carcinoma cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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