7162930
PCBP2 (hnRNP E2), Mouse anti-Human, Clone: PC 81-1, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
PCBP2 (hnRNP E2)
Dilution
Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).Western Blotting Analysis: 1μg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Poly(rC)-binding protein 2;Alpha-CP2;Heterogeneous nuclear ribonucleoprotein E2;hnRNP E2
Gene Symbols
PCBP2
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
Clone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
Clone
PC 81-1
Applications
Immunocytochemistry, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001122383
Immunogen
His-tagged full-length human recombinant Poly(rC)-binding protein 2.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-PCBP2 (hnRNP E2), clone PC81-1, Cat
- No
- MABF2122, is a mouse monoclonal antibody that detects Poly(rC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting
- Poly(rC)-binding protein 2 (UniProt: Q15366; also known as Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2) is encoded by the PCBP2 gene (Gene ID: 5094) in human
- PCBP2 belongs to a class of cellular proteins that bind to poly(C) stretches of both RNA and DNA and has been detected in all tissues studied
- PCBP2 shuttles between the nucleus and cytoplasm and exerts its impact on RNA processing and mRNA expression through sequence-specific interactions with C-rich determinants within target mRNAs
- It acts as an important mediator of multiple processes, including replication of viruses with gastrointestinal and hepatic tropism, hepatic collagen synthesis, globin expression, and cellular proliferation
- PCBP2 is shown to negatively regulate cellular antiviral responses mediated by mitochondrial antiviral-signaling protein (MAVS) signaling and acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, thereby triggering MAVS ubiquitination and degradation
- Together with PCBP1, it is shown to function as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication
- It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA
- PCBP2 contains three K-homologous (KH) domains that mediates poly(C) binding
- Eight different isoforms of PCBP2 have been described that are produced by alternative splicing
- (Ref.: Ghanem, LR et al
- (2014)
- Gene Exp
- Patterns 14(2); 78-87; Sean, P., et al
- (2008)
- Virology
- Virology
- 378(2); 243-253).
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Antigen: PCBP2 (hnRNP E2)
Dilution: Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).Western Blotting Analysis: 1μg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Poly(rC)-binding protein 2;Alpha-CP2;Heterogeneous nuclear ribonucleoprotein E2;hnRNP E2
Gene Symbols: PCBP2
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
Clone: PC 81-1
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001122383
Immunogen: His-tagged full-length human recombinant Poly(rC)-binding protein 2.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
PCBP2 (hnRNP E2)
Dilution:
Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).Western Blotting Analysis: 1μg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Poly(rC)-binding protein 2;Alpha-CP2;Heterogeneous nuclear ribonucleoprotein E2;hnRNP E2
Gene Symbols:
PCBP2
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
Clone:
PC 81-1
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001122383
Immunogen:
His-tagged full-length human recombinant Poly(rC)-binding protein 2.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: chlamydial MOMP
Dilution: Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81). Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia major outer membrane protein
Gene Symbols: N/A
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.
Clone: B-D3
Applications: Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez): __
Immunogen: Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
chlamydial MOMP
Dilution:
Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81). Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia major outer membrane protein
Gene Symbols:
N/A
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.
Clone:
B-D3
Applications:
Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez):
__
Immunogen:
Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Aminopeptidase N (CD13)
Dilution: Immunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. al. (2014). Front Physiol. 4:402; Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. al. (2014). Front Physiol. 4:402).Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: AP-N;hAPN;Alanyl aminopeptidase;Aminopeptidase M;AP-M;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13;gp150;CD13
Gene Symbols: ANPEP;APN;CD13;PEPN
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 452 specifically detects Aminopeptidase N (CD13) in human cells.
Clone: 452
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez): NP_001141
Immunogen: Isolated human dermal fibroblasts.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Aminopeptidase N (CD13)
Dilution:
Immunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. al. (2014). Front Physiol. 4:402; Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. al. (2014). Front Physiol. 4:402).Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
AP-N;hAPN;Alanyl aminopeptidase;Aminopeptidase M;AP-M;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13;gp150;CD13
Gene Symbols:
ANPEP;APN;CD13;PEPN
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 452 specifically detects Aminopeptidase N (CD13) in human cells.
Clone:
452
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez):
NP_001141
Immunogen:
Isolated human dermal fibroblasts.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: TMPRSS2
Dilution: Immunohistochemistry (Paraffin) Analysis: 1:50 dilution from a representative lot detected TMPRSS2 in human kidney tissue sections.Enzyme Immunoassay (ELISA) Analysis: A representative lot detected TMPRSS2 in ELISA applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Western Blotting Analysis: A representative lot detected TMPRSS2 in Western Blotting applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Immunohistochemistry (Paraffin) Analysis: A representative lot detected TMPRSS2 in Immunohistochemistry applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25; Bertram, S., et. al. (2012). PLoS One. 7(4):e35876).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Transmembrane protease serine 2;Serine protease 10
Gene Symbols: TMPRSS2;PRSS10
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone P5H9-A3 specifically detects human Transmembrane protease serine 2. It targets an epitope with in the extracellular serine protease domain.
Clone: P5H9-A3
Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human transmembrane protease serine 2.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
TMPRSS2
Dilution:
Immunohistochemistry (Paraffin) Analysis: 1:50 dilution from a representative lot detected TMPRSS2 in human kidney tissue sections.Enzyme Immunoassay (ELISA) Analysis: A representative lot detected TMPRSS2 in ELISA applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Western Blotting Analysis: A representative lot detected TMPRSS2 in Western Blotting applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Immunohistochemistry (Paraffin) Analysis: A representative lot detected TMPRSS2 in Immunohistochemistry applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25; Bertram, S., et. al. (2012). PLoS One. 7(4):e35876).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Transmembrane protease serine 2;Serine protease 10
Gene Symbols:
TMPRSS2;PRSS10
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone P5H9-A3 specifically detects human Transmembrane protease serine 2. It targets an epitope with in the extracellular serine protease domain.
Clone:
P5H9-A3
Applications:
ELISA, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human transmembrane protease serine 2.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.