7162972
ADAM 12, Mouse anti-Human, Clone: 8F8, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
ADAM 12
Dilution
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols
ADAM12;MLTN;UNQ346/PRO545
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Clone
8F8
Applications
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Apoptosis & Cancer
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001275903
Immunogen
A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-ADAM 12, clone 8F8, Cat
- No
- MABT1330, is a mouse monoclonal antibody that detects Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, and Western Blotting
- Disintegrin and metalloproteinase domain-containing protein 12 (UniProt: O43184, also known as ADAM 12, Meltrin-alpha) is encoded by the ADAM12 (also known as MLTN, UNQ346/PRO545) gene (Gene ID: 8038) in human
- ADAM (A disintegrin and metalloprotease) family consists of multidomain cell-surface proteins that are synthesized in their proform
- Four isoforms of ADAM12 have been described that are produced by alternative splicing
- Isoform 1 is a single-pass transmembrane protein and isoforms 2,3, and 4 are secreted proteins
- ADM12 is synthesized with a signal peptide (aa 1-28) and a propeptide (aa 29-207), which are subsequently cleaved off in the mature form
- Isoform 1 shown to be expressed in placenta and in skeletal, cardiac, and smooth muscle
- Isoform 2 is reported to be expressed only in placenta or in embryo and fetus
- ADAM12 contains a cysteine rich domain (aa 514-649) that support cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading
- However, in carcinoma cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading
- The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme
- Dissociation of the cysteine from the zinc ion activates the enzyme
- Upregulation of ADAM12 has been reported in malignant tumors
- In human breast cancer cells expressing ADAM12, MMP-14 is shown to be recruited to the cell surface which results in ADAM12 activation and accelerates tumor progression
- ADAM12 does not affect tumor cell proliferation, but it causes a diminution in tumor cell apoptosis and increases stromal cell apoptosis
- (Ref.: Sundberg, C., et al
- (2004)
- J
- Biol
- Chem
- 279(49); 51601 51611; Albrechtsen, R., et al
- (2013)
- J
- Cell Sci
- 126(20); 4707 4720; Kveiborg, M., et al
- (2005)
- Cancer Res
- 65(11); 4754-4761).
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Antigen: ADAM 12
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Clone: 8F8
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis & Cancer
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001275903
Immunogen: A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ADAM 12
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Clone:
8F8
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis & Cancer
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001275903
Immunogen:
A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ADAM 12
Dilution: Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Clone: 6E6
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis & Cancer
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001275903
Immunogen: A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ADAM 12
Dilution:
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Clone:
6E6
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis & Cancer
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001275903
Immunogen:
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: pan-Actin
Dilution: Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Gene Alias: Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Gene Symbols: ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Clone: 2A3
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001092
Immunogen: KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
pan-Actin
Dilution:
Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Gene Alias:
Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Gene Symbols:
ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Clone:
2A3
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001092
Immunogen:
KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Lamin A/C
Dilution: Immunoprecipitation Analysis: A representative immunoprecipitated Lamin A/C in culture supernatant (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Western Blotting Analysis: A representative lot detected Lamin A/C in culture supernatants of various cell lines (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells. Immunofluorescence Analysis: A representative lot detected Lamin A/C in the nuclear interior of HeLa cells (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Monkey, Hamster, Mouse
Gene Alias: 70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols: LMNA;LMN1
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 2A1 detects the Lamin A/C species in the nuclear interior in multiple species. It targets the Rod domain of Lamin A/C.
Clone: 2A1
Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_733821
Immunogen: Purified protein corresponding to the rod domain for human Lamin A/C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Lamin A/C
Dilution:
Immunoprecipitation Analysis: A representative immunoprecipitated Lamin A/C in culture supernatant (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Western Blotting Analysis: A representative lot detected Lamin A/C in culture supernatants of various cell lines (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells. Immunofluorescence Analysis: A representative lot detected Lamin A/C in the nuclear interior of HeLa cells (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Monkey, Hamster, Mouse
Gene Alias:
70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols:
LMNA;LMN1
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 2A1 detects the Lamin A/C species in the nuclear interior in multiple species. It targets the Rod domain of Lamin A/C.
Clone:
2A1
Applications:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_733821
Immunogen:
Purified protein corresponding to the rod domain for human Lamin A/C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.