7162974
pan-Actin, Mouse anti-Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast, Clone: 2A3, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
pan-Actin
Dilution
Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Gene Alias
Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Gene Symbols
ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Clone
2A3
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Structure
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001092
Immunogen
KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-pan-Actin, clone 2A3, Cat
- No
- MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting
- Actin is a multi-functional cytoskeletal protein that serves as the basic building block of cytoskeletal microfilaments
- Six different actin types have been reported
- Skeletal muscle alpha-actin (UniProt: P68133) is encoded by the ACTA1 gene, smooth muscle alpha-actin (UniProt: P62736) by the ACTA2 gene, cytoplasmic beta-actin (UniProt: P60709) by the ACTB gene, cardiac muscle alpha-actin (UniProt: P68032) by the ACTC1 gene, cytoplasmic gamma-actin (UniProt: P63261) by the ACTG1 gene, and smooth muscle gamma-actin (UniProt: P63267) by the ACTG2 (also known as ACTA3) gene
- Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues
- Cytoplasmic beta and gamma actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types
- Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins
- The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt
- This pan-actin antibody detects all isoforms actin.
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Antigen: pan-Actin
Dilution: Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Gene Alias: Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Gene Symbols: ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Clone: 2A3
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001092
Immunogen: KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
pan-Actin
Dilution:
Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Gene Alias:
Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Gene Symbols:
ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Clone:
2A3
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001092
Immunogen:
KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Lamin A/C
Dilution: Immunoprecipitation Analysis: A representative immunoprecipitated Lamin A/C in culture supernatant (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Western Blotting Analysis: A representative lot detected Lamin A/C in culture supernatants of various cell lines (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells. Immunofluorescence Analysis: A representative lot detected Lamin A/C in the nuclear interior of HeLa cells (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Monkey, Hamster, Mouse
Gene Alias: 70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols: LMNA;LMN1
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 2A1 detects the Lamin A/C species in the nuclear interior in multiple species. It targets the Rod domain of Lamin A/C.
Clone: 2A1
Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_733821
Immunogen: Purified protein corresponding to the rod domain for human Lamin A/C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Lamin A/C
Dilution:
Immunoprecipitation Analysis: A representative immunoprecipitated Lamin A/C in culture supernatant (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Western Blotting Analysis: A representative lot detected Lamin A/C in culture supernatants of various cell lines (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells. Immunofluorescence Analysis: A representative lot detected Lamin A/C in the nuclear interior of HeLa cells (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Monkey, Hamster, Mouse
Gene Alias:
70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols:
LMNA;LMN1
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 2A1 detects the Lamin A/C species in the nuclear interior in multiple species. It targets the Rod domain of Lamin A/C.
Clone:
2A1
Applications:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_733821
Immunogen:
Purified protein corresponding to the rod domain for human Lamin A/C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Lamin A/C
Dilution: Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunoprecipitation Analysis: A representative lot de
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Monkey, Syrian Hamster, Rat, Mouse, Human
Gene Alias: 70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols: LMNA;LMN1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Clone: 4C11
Applications: ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001244303
Immunogen: Purified protein corresponding to the Ig-fold of human Lamin A/C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Lamin A/C
Dilution:
Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunoprecipitation Analysis: A representative lot de
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Monkey, Syrian Hamster, Rat, Mouse, Human
Gene Alias:
70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols:
LMNA;LMN1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Clone:
4C11
Applications:
ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001244303
Immunogen:
Purified protein corresponding to the Ig-fold of human Lamin A/C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Lamin A/C
Dilution: Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunoprecipitation Analysis: A representative lot de
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Monkey, Syrian Hamster, Rat, Mouse, Human
Gene Alias: 70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols: LMNA;LMN1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Clone: 4C11
Applications: ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001244303
Immunogen: Purified protein corresponding to the Ig-fold of human Lamin A/C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Lamin A/C
Dilution:
Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunoprecipitation Analysis: A representative lot de
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Monkey, Syrian Hamster, Rat, Mouse, Human
Gene Alias:
70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols:
LMNA;LMN1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Clone:
4C11
Applications:
ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001244303
Immunogen:
Purified protein corresponding to the Ig-fold of human Lamin A/C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.