7162976
Lamin A/C, Mouse anti-Monkey, Syrian Hamster, Rat, Mouse, Human, Clone: 4C11, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Lamin A/C
Dilution
Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunoprecipitation Analysis: A representative lot de
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Monkey, Syrian Hamster, Rat, Mouse, Human
Gene Alias
70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols
LMNA;LMN1
Isotype
IgG2a κ
Purification Method
Protein G purified
Test Specificity
Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Clone
4C11
Applications
ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Structure
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001244303
Immunogen
Purified protein corresponding to the Ig-fold of human Lamin A/C.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Lamin A/C, clone 4C11, Cat
- No
- MABT1341, is a highly specific mouse monoclonal antibody that targets Lamin A/C and has been tested for use in Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), and Western Blotting
- Prelamin-A/C (UniProt: P02545) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human
- It is cleaved into Lamin-A/C (also known as 70 kDa Lamin, Renal carcinoma antigen NY-REN-32)
- Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin
- Lamin A and C are present in equal amounts in the lamina of mammals
- Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics
- Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A
- Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product
- Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery
- Prelamin-A/C can accelerate smooth muscle cell senescence
- It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence
- Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system
- Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body
- (Ref.: Casasola, A., et al
- (2016)
- Nucleus 7(1); 84-102).
Compare Similar Items
Show Difference
Antigen: Lamin A/C
Dilution: Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunoprecipitation Analysis: A representative lot de
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Monkey, Syrian Hamster, Rat, Mouse, Human
Gene Alias: 70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols: LMNA;LMN1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Clone: 4C11
Applications: ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001244303
Immunogen: Purified protein corresponding to the Ig-fold of human Lamin A/C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Lamin A/C
Dilution:
Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).Immunoprecipitation Analysis: A representative lot de
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Monkey, Syrian Hamster, Rat, Mouse, Human
Gene Alias:
70 kDa Lamin;Renal carcinoma antigen NY-REN-32
Gene Symbols:
LMNA;LMN1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Clone:
4C11
Applications:
ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001244303
Immunogen:
Purified protein corresponding to the Ig-fold of human Lamin A/C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Tropomyosin-1
Dilution: Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Human
Gene Alias: Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1
Gene Symbols: TPM1;C15orf13;TMSA
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
Clone: 7F8B8
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Cell Structure
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000357.3
Immunogen: KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Tropomyosin-1
Dilution:
Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Human
Gene Alias:
Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1
Gene Symbols:
TPM1;C15orf13;TMSA
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
Clone:
7F8B8
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Cell Structure
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_000357.3
Immunogen:
KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CRB3A
Dilution: Western Blotting Analysis: 2μg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Protein crumbs homolog 3
Gene Symbols: CRB3;UNQ588;PRO1158
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.
Clone: 1E6
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Cell Structure
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_631900
Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CRB3A
Dilution:
Western Blotting Analysis: 2μg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Protein crumbs homolog 3
Gene Symbols:
CRB3;UNQ588;PRO1158
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.
Clone:
1E6
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Cell Structure
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_631900
Immunogen:
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Kinesin Light Chain
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.Western Blotting Analysis: 4μg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat, Mouse, Squid
Gene Alias: KLC1;KLC2;KLC
Gene Symbols: Klc1;Klc;Kns2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.
Clone: 63-90
Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001075443
Immunogen: Purified rat brain Kinesin light chain 1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Kinesin Light Chain
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.Western Blotting Analysis: 4μg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat, Mouse, Squid
Gene Alias:
KLC1;KLC2;KLC
Gene Symbols:
Klc1;Klc;Kns2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.
Clone:
63-90
Applications:
Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001075443
Immunogen:
Purified rat brain Kinesin light chain 1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.