7162980
Kinesin Light Chain, Mouse anti-Rat, Mouse, Squid, Clone: 63-90, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Kinesin Light Chain
Dilution
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.Western Blotting Analysis: 4μg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Rat, Mouse, Squid
Gene Alias
KLC1;KLC2;KLC
Gene Symbols
Klc1;Klc;Kns2
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.
Clone
63-90
Applications
Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Structure
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001075443
Immunogen
Purified rat brain Kinesin light chain 1.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Kinesin Light Chain, clone 63-90, Cat
- No
- MABT1489, is a mouse monoclonal antibody that detects Kinesin light chain and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting
- Kinesin light chain (UniProt: P37285 and B2GV74 for KLC1 and KLC2, respectively) is encoded by the Klc1/Klc2 (also known as Klc, Kns2) gene (Gene ID: 171041/309159) in rat
- Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport
- The kinesin light chain (KLC) may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity
- Kinesin light chain 1 is expressed in the brain tissue and exists as an oligomeric complex of two heavy and two light chains
- It also contains six tetratricopeptide repeats (TPR)
- The heavy chains of kinesin have a microtubule-binding ATPase motor domain at their amino terminus, a neck coil, and an extended series of coiled coils, separated by a hinge region, that results in heavy-chain dimerization
- Kinesin light chains can associate with the heavy-chain coiled coils at the carboxyl-terminal region
- The light chains bind cargoes and regulate its activity
- KLCs through their TRP repeat domains can recognize shirt linear peptide motifs found on cargo proteins
- In the absence of any cargo binding, kinesin-1 exists in a folded compact state, which prevents unnecessary cycles of ATP hydrolysis
- However, in the cargo-bound active state inhibition is relieved and it adopts a more elongated structure that can hydrolyze ATP and translocate along microtubules
- Point mutations in Klc gene can affect functional domains in kinesin, which can result in late-onset dying-back neuropathies in sensory or motor neurons
- (Ref.: Yip, YY., et al
- (2016)
- Proc
- Natl
- Acad
- Sci
- USA 113(9); 2418-2423; Pigino, G., et al
- (2009)
- Proc
- Natl
- Acad
- Sci
- USA 106(14); 5907-5912).
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Antigen: Kinesin Light Chain
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.Western Blotting Analysis: 4μg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat, Mouse, Squid
Gene Alias: KLC1;KLC2;KLC
Gene Symbols: Klc1;Klc;Kns2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.
Clone: 63-90
Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001075443
Immunogen: Purified rat brain Kinesin light chain 1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Kinesin Light Chain
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.Western Blotting Analysis: 4μg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat, Mouse, Squid
Gene Alias:
KLC1;KLC2;KLC
Gene Symbols:
Klc1;Klc;Kns2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.
Clone:
63-90
Applications:
Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001075443
Immunogen:
Purified rat brain Kinesin light chain 1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: OTK18(ZNF175)
Dilution: ELISA Analysis: A representative lot detected OTK18(ZNF175) in ELISA applications (Buescher, J.L., et. al. (2008). J Neuroimmune Pharmacol. 3(4):230-5).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Zinc finger protein OTK18
Gene Symbols: ZNF175
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 211 3.1 1 specifically detects human Zinc finger protein 175. It targets an epitope with in the first 178 amino acids from the N-terminal region.
Clone: 211−3.1−1
Applications: ELISA, Immunocytochemistry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_009078.1
Immunogen: His-tagged recombinant fragment corresponding to the first 178 amino acids from the N-terminal region of human Zinc finger protein 175.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
OTK18(ZNF175)
Dilution:
ELISA Analysis: A representative lot detected OTK18(ZNF175) in ELISA applications (Buescher, J.L., et. al. (2008). J Neuroimmune Pharmacol. 3(4):230-5).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Zinc finger protein OTK18
Gene Symbols:
ZNF175
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 211 3.1 1 specifically detects human Zinc finger protein 175. It targets an epitope with in the first 178 amino acids from the N-terminal region.
Clone:
211−3.1−1
Applications:
ELISA, Immunocytochemistry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_009078.1
Immunogen:
His-tagged recombinant fragment corresponding to the first 178 amino acids from the N-terminal region of human Zinc finger protein 175.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Desmoplakin
Dilution: Western Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: DP;250/210 kDa paraneoplastic pemphigus antigen
Gene Symbols: DSP
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
Clone: 20B6
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001008844
Immunogen: His-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Desmoplakin
Dilution:
Western Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
DP;250/210 kDa paraneoplastic pemphigus antigen
Gene Symbols:
DSP
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
Clone:
20B6
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001008844
Immunogen:
His-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Desmoplakin
Dilution: Western Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: DP;250/210 kDa paraneoplastic pemphigus antigen
Gene Symbols: DSP
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
Clone: 20B6
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001008844
Immunogen: His-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Desmoplakin
Dilution:
Western Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
DP;250/210 kDa paraneoplastic pemphigus antigen
Gene Symbols:
DSP
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
Clone:
20B6
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001008844
Immunogen:
His-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.