7162979
CRB3A, Rat anti-Human, Clone: 1E6, Millipore Sigma™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CRB3A
Dilution
Western Blotting Analysis: 2μg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Protein crumbs homolog 3
Gene Symbols
CRB3;UNQ588;PRO1158
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.
Clone
1E6
Applications
Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Host Species
Rat
Research Discipline
Cell Structure
Formulation
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_631900
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-CRB3A, clone 1E6, Cat
- No
- MABT1366, is a rat monoclonal antibody that detects Protein crumbs homolog 3 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
- Protein crumbs homolog 3 (UniProt: Q9BUF7) is encoded by the CRB3 (also known as UNQ588/PRO1158) gene (Gene ID: 92359) in human
- Mammalian cells contain three Crb orthologs
- CRB1 is primarily expressed in the brain, cornea, and retina
- CRB2 is expressed in lung, kidney, retina, colon and mammary glands
- It is also expressed in at moderate levels in Expressed at moderate levels in liver, spleen, pancreas, placenta, and prostate tissue
- CRB3 is present in epithelial tissues and is present in two isoforms, CRB3A and CRB3B
- CRB3 is a single-pass type I membrane protein that is reported to control epithelial cell polarization
- CRB3A is apically localized and promotes apical membrane growth and is and is essential for the formation of tight junctions in epithelial cells
- It also prevents the epithelial-to-mesenchymal transition (EMT), which is known to contribute to tumor progression
- CRB3 is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off
- The mature form contains an extracellular domain (aa 27-59), a transmembrane domain (aa 60-80), and a cytoplasmic tail (aa 81-120), which contains a PDZ-domain binding motif (aa 117-120) that interacts with the Maguk protein, PALS1, which recruits PALS1-associated tight junction (PATJ) protein into the CRB3A complex
- Both PALS1 and PATJ play a critical role in epithelial cell polarity and tight junction formation
- CRB3A is shown to control and properly localize RhoA signaling that is required for CRB3A-dependent reorganization of the actin cytoskeleton
- (Ref.: Loie, E., et al (2015)
- Mol
- Cell Biol
- 35(19); 3423-3435).
Compare Similar Items
Show Difference
Antigen: CRB3A
Dilution: Western Blotting Analysis: 2μg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Protein crumbs homolog 3
Gene Symbols: CRB3;UNQ588;PRO1158
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.
Clone: 1E6
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Cell Structure
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_631900
Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CRB3A
Dilution:
Western Blotting Analysis: 2μg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Protein crumbs homolog 3
Gene Symbols:
CRB3;UNQ588;PRO1158
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.
Clone:
1E6
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Cell Structure
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_631900
Immunogen:
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Kinesin Light Chain
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.Western Blotting Analysis: 4μg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat, Mouse, Squid
Gene Alias: KLC1;KLC2;KLC
Gene Symbols: Klc1;Klc;Kns2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.
Clone: 63-90
Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001075443
Immunogen: Purified rat brain Kinesin light chain 1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Kinesin Light Chain
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.Western Blotting Analysis: 4μg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat, Mouse, Squid
Gene Alias:
KLC1;KLC2;KLC
Gene Symbols:
Klc1;Klc;Kns2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.
Clone:
63-90
Applications:
Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001075443
Immunogen:
Purified rat brain Kinesin light chain 1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: OTK18(ZNF175)
Dilution: ELISA Analysis: A representative lot detected OTK18(ZNF175) in ELISA applications (Buescher, J.L., et. al. (2008). J Neuroimmune Pharmacol. 3(4):230-5).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Zinc finger protein OTK18
Gene Symbols: ZNF175
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 211 3.1 1 specifically detects human Zinc finger protein 175. It targets an epitope with in the first 178 amino acids from the N-terminal region.
Clone: 211−3.1−1
Applications: ELISA, Immunocytochemistry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_009078.1
Immunogen: His-tagged recombinant fragment corresponding to the first 178 amino acids from the N-terminal region of human Zinc finger protein 175.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
OTK18(ZNF175)
Dilution:
ELISA Analysis: A representative lot detected OTK18(ZNF175) in ELISA applications (Buescher, J.L., et. al. (2008). J Neuroimmune Pharmacol. 3(4):230-5).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Zinc finger protein OTK18
Gene Symbols:
ZNF175
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 211 3.1 1 specifically detects human Zinc finger protein 175. It targets an epitope with in the first 178 amino acids from the N-terminal region.
Clone:
211−3.1−1
Applications:
ELISA, Immunocytochemistry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_009078.1
Immunogen:
His-tagged recombinant fragment corresponding to the first 178 amino acids from the N-terminal region of human Zinc finger protein 175.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Desmoplakin
Dilution: Western Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: DP;250/210 kDa paraneoplastic pemphigus antigen
Gene Symbols: DSP
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
Clone: 20B6
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001008844
Immunogen: His-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Desmoplakin
Dilution:
Western Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
DP;250/210 kDa paraneoplastic pemphigus antigen
Gene Symbols:
DSP
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
Clone:
20B6
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001008844
Immunogen:
His-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.