phospho-cytokeratin-8 (K8) (Ser73), Mouse anti-Mouse, Human, Clone: LJ4, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
phospho-cytokeratin-8 (K8) (Ser73)
Dilution
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse, Human
Gene Alias
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Gene Symbols
KRT8;CYK8
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Clone
LJ4
Applications
ELISA, Immunocytochemistry, Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Structure
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001243222
Immunogen
Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-phospho-cytokeratin-8 (K8) (Ser73), clone LJ4, Cat
- No
- MABT344, is a mouse monoclonal antibody that detects human type II cytoskeletal 8 phophrorylated on serine 73 and murine cytokeratin-8 phophorylated on serine 79 and has been tested for use in ELISA, Immunocytochemistry, Immunofluoresce Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8 Keratin-8, K8, Type-II keratin Kb80) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human
- Cytokeratin-8 is a type II, neutral to basic, protein of the intermediate filament family that together with Cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle
- Cytokeratin-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73
- Serine 23 is shown to be highly conserved in all type II keratins
- Phosphorylation at Serine 73 is reported to increase during cellular stress, including hear and drug exposure
- However, under normal conditions serine 73 remains largely dephosphorylated
- Cytokeratin-8 can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation
- Mutations in KRT8 gene have been linked to liver cirrhosis that is characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper
- Two isoforms of Cytokeratin-8 have been described that are produced by alternative splicing.
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Antigen: phospho-cytokeratin-8 (K8) (Ser73)
Dilution: Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Human
Gene Alias: Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Gene Symbols: KRT8;CYK8
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Clone: LJ4
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001243222
Immunogen: Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen: phospho-cytokeratin-18 (K18) (Ser33)
Dilution: Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-18 (K18) (Ser33) in immunocytochemistry applications (Ku, N.O., et. al. (1998). EMBO J. 17(7):1892-906).Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-cytokeratin-18 (K18) (Ser33) in immunoprecipitation applications (Ku, N.O., et. al. (1998). EMBO J. 17(7):1892-906).Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-18 (K18) (Ser33) in Immunofluorescence applications (Ku, N.O., et. al. (1998). EMBO J. 17(7):1892-906).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-18 (K18) (Ser33) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Keratin type I cytoskeletal 18;Cell proliferation-inducing gene 46 protein;CK-18;Keratin-18;K18
Gene Symbols: KRT18;CYK18;PIG46
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone IB4 specifically detects human cytokeratin-18 phosphorylated on serine 33.
Clone: IB4
Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000215
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphorylated serine 33 (initiator methionine removed) from the N-terminal region of human cytokeratin-18.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen: Tspan-33
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Human
Gene Alias: Tetraspanin-33;Penumbra;mPen;Proerythroblast new membrane
Gene Symbols: Tspan33;Pen
Isotype: IgM κ
Purification Method: Protein G purified
Test Specificity: Clone 29A6.2 specifically detects Tspan-33 and targets an epitope with in the extracellular domain.
Clone: 29A6.2
Applications: Flow Cytometry
Conjugate: FITC
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgM in PBS with 0.1% sodium azide, with 1.5% BSA.
Gene ID (Entrez): NP_666285.1
Immunogen: BaF3 cells expressing full-length murine Tspan-33 fused with a Myc-His tag at the C-terminus.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
