Invitrogen™ LanthaScreen™ Tb-anti-GFP Antibody

Description

• LanthaScreen™ Tb anti-GFP Antibody is a terbium-labeled antibody recognizing green fluorescent protein (GFP)
• It can be used to determine GFP expression levels in any GFP expressing cell line with greater sensitivity than detecting the fluorescence intensity of the GFP protein alone
• With the LanthaScreen™ Tb anti-GFP Antibody You Can: Amplify GFP signal expression for more sensitive results Track GFP-target protein expression levels Easily build TR-FRET assays from any GFP expressing cell line Detect Low Level GFP Expression GFP is a commonly used reporter for many types of research
• In some instances, the level of GFP-target protein expression is not high enough to generate a suitably strong fluorescence intensity signal
• The LanthaScreen™ Tb anti-GFP Antibody can help overcome this problem through the use of TR-FRET assays
• Since TR-FRET is a ratiometric readout and does not rely on the intensity of the signal alone, low levels of GFP expression are not a problem
• Use TR-FRET to Track Protein Expression Levels Using the LanthaScreen™ Tb anti-GFP Antibody in conjunction with a cell line expressing GFP-target fusion protein can allow development of TR-FRET assays for protein accumulation or degradation
• Following titration of a compound that induces or inhibits the expression of your GFP-target protein, LanthaScreen™ Tb anti-GFP can be added to the resultant cell lysates and the TR-FRET ratios compared to determine the relative effect of the compound on the GFP-target protein expression level
• Create New TR-FRET Assays from Any GFP Expressing Cell Line Because the terbium label on the LanthaScreen™ Tb anti-GFP Antibody can be used as a donor for TR-FRET with GFP, any GFP-expressing cell line can be used with the antibody to build a TR-FRET assay
• The antibody will bind to GFP in a cell lysate sample, and a simple ratiometric measurement can determine the differences in GFP expression levels between different samples.