Phospho-ATG13 (Ser318) Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity-purified antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue
• Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein
• Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot
• A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen
• Reactivity against homologues from other sources is not known
• Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells
• It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components
• This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1
• ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200
• This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy
• Knockdown of ATG13 inhibits autophagosome formation.