Sendai virus HN Monoclonal Antibody (1A6), Alexa Fluor™ 647, eBioscience™, Invitrogen™

Description

• Description: This 1A6 monoclonal antibody recognizes hemagglutinin-neuraminidase of Sendai virus
• This clone has been successfully used in flow cytometry, immunocytochemistry and has been reported for neutralization
• The CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit and the CytoTune-iPS 2.0 Sendai Reprograming Kit utilize Sendai virus particles to deliver Yamanaka factors into target cells in order to reprogram them into induced Pluripotent Stem Cells (iPSC)
• Clone 1A6 can be used to detect the presence of the residual Sendai virus after transduction and subsequent tissue culture
• Applications Reported: This 1A6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1A6 antibody has been tested by flow cytometric analysis of Sendai virus-infected cells
• This may be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser Sendai virus is a single stranded RNA respirovirus from the family Paramyxoviridae, which replicates in the respiratory tract and infects certain mammals, including mice, hamsters, rats and guinea pigs
• The virus is highly contagious and in the wild is transmitted through contact with aerosolized respiratory secretions
• The Hemagglutinin-Neuraminidase (HN) protein of the Sendai virus facilitates cell entry and infection by binding and cleaving terminal sialic acids present on many cell surface glycoproteins.