CD162 (PSGL-1) Monoclonal Antibody (4RA10), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1)
• This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L
• Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets
• CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin)
• These interactions play a critical role in leukocyte adhesion, rolling and extravasation
• CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.