7193270

CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor™ 647, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD4

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias

Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen

Gene Symbols

CD4

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

CD4

Clone

RPA-T4

Applications

Flow Cytometry

Conjugate

Alexa Fluor 647

Host Species

Mouse

Target Species

Human

Gene Accession No.

P01730

Gene ID (Entrez)

920

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes
  • CD4 is a receptor for the human immunodeficiency virus (HIV)
  • RPA-T4 blocks HIV binding and mixed lymphocyte reaction
  • The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes
  • Applications Reported: This RPA-T4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This RPA-T4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
  • This may be used at 5 μL (0.25 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
  • The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
  • CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
  • At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
  • CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
  • It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
  • Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

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Fischer Scientific

7193270

Mouse Monoclonal Antibody...


Antigen:
CD4

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen

Gene Symbols:
CD4

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD4

Clone:
RPA-T4

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 647

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P01730

Gene ID (Entrez):
920

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7193281

Rat Monoclonal Antibody...


Antigen:
MHC Class II (I-A/I-E)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1

Gene Symbols:
H2-Ab1, H2-Eb1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
H2-Ab1

Clone:
M5/114.15.2

Applications:
Flow Cytometry

Conjugate:
eFluor 506

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P01921, P04230, P06342, P06343, P06344, P06345, P06346, P14483

Gene ID (Entrez):
14961, 14969

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7193282

Mouse Monoclonal Antibody...


Antigen:
CD43

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
3E8 antigen; A630014B01Rik; B-cell differentiation antigen LP-3; CD43; CD43 cytoplasmic tail; CD43-ct; Galactoglycoprotein; GALGP; gpL115; Leukocyte sialoglycoprotein; leukosialin; leukosianin; LOC101120462; LOW QUALITY PROTEIN: leukosialin; Lsn; Lsn1; Ly48; Ly-48; Lymphocyte antigen 48; sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115; SPN; W3/13 antigen

Gene Symbols:
SPN

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
SPN

Clone:
4-29-5-10-21

Applications:
Flow Cytometry

Conjugate:
APC

Host Species:
Mouse

Target Species:
Human, Non-human Primate

Gene Accession No.:
P16150

Gene ID (Entrez):
6693

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7193285

Mouse Monoclonal Antibody...


Antigen:
CD42b

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
Antigen CD42b-alpha; BDPLT1; BDPLT3; BSS; CD42a; CD42B; CD42b-alpha; DBPLT3; Glycocalicin; glycoprotein 1b, alpha polypeptide; glycoprotein Ib (platelet), alpha polypeptide; glycoprotein Ib platelet alpha subunit; glycoprotein Ib platelet subunit alpha; glycoprotein Ibalpha; GP1B; GP1BA; GPIB; GPIb alpha; GP-Ib alpha; GPIbA; GPIbalpha; GPIX; GP-IX; MGC34595; mutant platelet membrane glycoprotein Ib-alpha; platelet glycoprotein Ib alpha chain; platelet glycoprotein Ib alpha polypeptide; Platelet GPIX; platelet membrane glycoprotein 1b-alpha subunit; platelet membrane glycoprotein Ib-alpha; VWDP

Gene Symbols:
Gp1ba

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Gp1ba

Clone:
HIP1

Applications:
Flow Cytometry

Conjugate:
Super Bright 600

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P07359

Gene ID (Entrez):
2811

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody