Applications:
 Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
 
 
 
 Gene Alias:
 CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
 
 
 Purification Method:
 Protein G purified
 
 Test Specificity:
 NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).
 
 
 Dilution:
 Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:10-1:500
 
 Classification:
 Monoclonal
 
 
 Target Species:
 Mouse (Negative), Porcine (Negative), Human
 
 Immunogen:
 Recombinant PI-9 produced in P. pastoris.
 
 Primary or Secondary:
 Primary
 
 Content And Storage:
 Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.