Phospho-Aurora A/B/C (Thr288, Thr232, Thr198) Recombinant Rabbit Monoclonal Antibody (AuroraABC-CC12), FITC, Invitrogen™

Description

• Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis
• The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly
• Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis
• Required for central/midzone spindle assembly and cleavage furrow formation
• Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515)
• AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP
• Phosphorylation of INCENP leads to increased AURKB activity
• Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3
• A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres
• Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively)
• A positive feedback between GSG2 and AURKB contributes to CPC localization
• AURKB is also required for kinetochore localization of BUB1 and SGOL1
• Phosphorylation of p53/TP53 negatively regulates its transcriptional activity
• Key regulator of active promoters in resting B- and T-lymphocytes.