CD254 (RANK Ligand) Monoclonal Antibody (MIH24), eBioscience™, Invitrogen™

Description

• Description: The MIH24 monoclonal antibody reacts with human TRANCE (TNF-related activation-induced cytokine receptor) also known as RANKL (receptor activator of NF-kappaB ligand) and OPGL (Osteoprotegerin Ligand)
• TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa
• Its extracellular domain is most closely related to TRAIL, FasL and TNF
• TRANCE mRNA is upregulated by TCR stimulation
• TRANCE-deficient mouse lacks osteoclasts
• In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells
• It was also found to be critical in osteoclast differentiation
• The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL
• Applications Reported: The MIH24 antibody has been reported for use in flow cytometric analysis, and ELISA
• Applications Tested: The MIH24 antibody has been tested by flow cytometric analysis of human RANKL transfected cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation
• This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response
• T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss
• This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis
• Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts
• The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy
• Two alternatively spliced transcript variants have been found.