CD69 Monoclonal Antibody (FN50), Alexa Fluor™ 561, eBioscience™, Invitrogen™

Description

• Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA)
• CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer
• CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets
• Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This FN50 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610
• Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions
• CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets
• In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction
• Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation
• Furthermore, the protein may act to transmit signals in natural killer cells and platelets
• Alternative splicing results in multiple transcript variants of CD69
• Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.