CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Ultra Violet™ 737, eBioscience™, Invitrogen™

Description

• The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197
• CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21
• CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane
• CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells
• CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue
• Expression of CCR7 is also found on dendritic cells
• During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation
• Expression of CCR7 on the cell surface can be down regulated upon ligand binding
• Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3D12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (1.0 μg) per test
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Brilliant Ultraviolet 737 is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser
• CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines
• CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes
• CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine
• CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation
• The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor
• ESTs have been isolated from blood, embryo, lymph node, and thymus libraries
• Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen
• The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.