7238646
Phosphoserine Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 3 publications
Manufacturer: Fischer Scientific
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Antigen
Phosphoserine
Dilution
Western Blot 1:500, ELISA 1:250, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10 - 1:500
Conjugate
Unconjugated
Regulatory Status
RUO
Gene Alias
Phosphoserine; Phosphothreonine
Isotype
IgG
Test Specificity
Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Concentration
0.25 mg/mL
Classification
Polyclonal
Host Species
Rabbit
Formulation
PBS, 50% Glycerol with 0.01 mg Sodium Azide
Immunogen
Phosphoserine conjugated to KLH, and phosvitin mixture
Primary or Secondary
Primary
Description
- Phosphoserine Polyclonal specifically detects Phosphoserine in Non-species specific samples
- It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.
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Antigen: Phosphoserine
Dilution: Western Blot 1:500, ELISA 1:250, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10 - 1:500
Conjugate: Unconjugated
Regulatory Status: RUO
Gene Alias: Phosphoserine; Phosphothreonine
Isotype: IgG
Test Specificity: Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Concentration: 0.25 mg/mL
Classification: Polyclonal
Host Species: Rabbit
Formulation: PBS, 50% Glycerol with 0.01 mg Sodium Azide
Immunogen: Phosphoserine conjugated to KLH, and phosvitin mixture
Primary or Secondary: Primary
Antigen:
Phosphoserine
Dilution:
Western Blot 1:500, ELISA 1:250, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10 - 1:500
Conjugate:
Unconjugated
Regulatory Status:
RUO
Gene Alias:
Phosphoserine; Phosphothreonine
Isotype:
IgG
Test Specificity:
Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Concentration:
0.25 mg/mL
Classification:
Polyclonal
Host Species:
Rabbit
Formulation:
PBS, 50% Glycerol with 0.01 mg Sodium Azide
Immunogen:
Phosphoserine conjugated to KLH, and phosvitin mixture
Primary or Secondary:
Primary
Antigen: Poly-Ubiquitin
Dilution: Western Blot 1:500 - 1:1000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100
Conjugate: Unconjugated
Regulatory Status: RUO
Gene Alias: CEP80, HEL112, ribosomal protein S27a, RPS27A, S27A, UBA80, UBC, UBCEP1, UBCEP80
Isotype: IgG2a
Test Specificity: Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.
Concentration: __
Classification: Monoclonal
Host Species: Mouse
Formulation: PBS with 0.09% Sodium Azide
Immunogen: The hybridoma secreting the antibody was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif.
Primary or Secondary: Primary
Antigen:
Poly-Ubiquitin
Dilution:
Western Blot 1:500 - 1:1000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100
Conjugate:
Unconjugated
Regulatory Status:
RUO
Gene Alias:
CEP80, HEL112, ribosomal protein S27a, RPS27A, S27A, UBA80, UBC, UBCEP1, UBCEP80
Isotype:
IgG2a
Test Specificity:
Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.
Concentration:
__
Classification:
Monoclonal
Host Species:
Mouse
Formulation:
PBS with 0.09% Sodium Azide
Immunogen:
The hybridoma secreting the antibody was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif.
Primary or Secondary:
Primary
Antigen: VISTA/B7-H5/PD-1H
Dilution: ELISA, Immunohistochemistry, Block/Neutralize
Conjugate: Unconjugated
Regulatory Status: RUO
Gene Alias: B7H5, B7-H5, C10orf54, chromosome 10 open reading frame 54, GI24, platelet receptor Gi24, PP2135, SISP1, stress induced secreted protein 1, VSIR
Isotype: IgG λ
Test Specificity: __
Concentration: __
Classification: Monoclonal
Host Species: Armenian Hamster
Formulation: PBS
Immunogen: The antibody was generated by immunising Armenian hamsters with mVISTA/B7-H5/PD-1HIg.
Primary or Secondary: Primary
Antigen:
VISTA/B7-H5/PD-1H
Dilution:
ELISA, Immunohistochemistry, Block/Neutralize
Conjugate:
Unconjugated
Regulatory Status:
RUO
Gene Alias:
B7H5, B7-H5, C10orf54, chromosome 10 open reading frame 54, GI24, platelet receptor Gi24, PP2135, SISP1, stress induced secreted protein 1, VSIR
Isotype:
IgG λ
Test Specificity:
__
Concentration:
__
Classification:
Monoclonal
Host Species:
Armenian Hamster
Formulation:
PBS
Immunogen:
The antibody was generated by immunising Armenian hamsters with mVISTA/B7-H5/PD-1HIg.
Primary or Secondary:
Primary
Antigen: B7-1/CD80
Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Block/Neutralize, Functional Assay
Conjugate: Unconjugated
Regulatory Status: RUO
Gene Alias: Activation B7-1 antigen, B7, BB1, CD28LG1, CD28LGB7-1 antigen), CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Isotype: IgG κ
Test Specificity: __
Concentration: 1 mg/mL
Classification: Monoclonal
Host Species: Rabbit
Formulation: PBS
Immunogen: This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
Primary or Secondary: Primary
Antigen:
B7-1/CD80
Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Block/Neutralize, Functional Assay
Conjugate:
Unconjugated
Regulatory Status:
RUO
Gene Alias:
Activation B7-1 antigen, B7, BB1, CD28LG1, CD28LGB7-1 antigen), CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Isotype:
IgG κ
Test Specificity:
__
Concentration:
1 mg/mL
Classification:
Monoclonal
Host Species:
Rabbit
Formulation:
PBS
Immunogen:
This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
Primary or Secondary:
Primary