CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Brilliant Violet™ 650, eBioscience™, Invitrogen™

Description

• The MEL-14 mAb reacts with mouse CD62L, a 76 kDa member of the selectin family
• CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1
• These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues
• Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.125ug per test BV 650 is a tandem dye that emits at 649nm and is intended for use on cytometers equipped with a violet (405nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, BV, BUV, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Excitation: 407nm; Emission: 649nm; Laser: Violet Laser
• BV is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
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• The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors
• L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region
• Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.