CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Brilliant Ultra Violet™ 496, eBioscience™, Invitrogen™

Description

• The MEL-14 mAb reacts with mouse CD62L, a 76 kDa member of the selectin family
• CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1
• These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues
• Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.125μg per test Brilliant Ultra Violet 496 (BUV496) is a tandem dye that emits at 496 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Excitation: 350 nm; Emission: 496 nm; Laser: Ultraviolet Laser
• The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors
• L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region
• Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.