CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet™ 661, eBioscience™, Invitrogen™

Description

• The ICRF44 mAb reacts with human CD11b, a 165 kDa adhesion molecule
• CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells
• CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b
• These adhesions are crucial in cell-cell and cell-matrix interactions
• Applications Reported: This ICRF44 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This ICRF44 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5μL (0.5μg) per test Brilliant Ultra Violet 661 (BUV661) is a tandem dye that emits at 660 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Excitation: 350 nm; Emission: 660 nm; Laser: Ultraviolet Laser
• CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18)
• The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3)
• CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain
• CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions
• CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue
• The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages
• Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages
• Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.