7245538

EGF Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

EGF

Applications

ELISA, Western Blot

Conjugate

Unconjugated

Host Species

Rabbit

Target Species

Human

Gene Accession No.

P01133

Gene ID (Entrez)

1950

Immunogen

E.coli-derived Recombinant Human EGF

Purification Method

Antigen affinity chromatography

Gene

EGF

Concentration

0.1-1.0 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Formulation

PBS with no preservative

Gene Alias

AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone

Gene Symbols

EGF

Primary or Secondary

Primary

Content And Storage

-20°C

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Description

  • AA Sequence of recombinant protein: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
  • Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EGF
  • Anti-Human EGF-specific antibody was purified by affinity chromatography employing an immobilized Human EGF matrix
  • Sandwich ELISA: To detect Human EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
  • This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EGF (500-P45Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EGF
  • Western Blot: To detect Human EGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
  • Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
  • EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase
  • Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members
  • Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins
  • EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands
  • EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades
  • EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules
  • Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade
  • Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

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7245538

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Antigen:
EGF

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Human

Gene Accession No.:
P01133

Gene ID (Entrez):
1950

Immunogen:
E.coli-derived Recombinant Human EGF

Purification Method:
Antigen affinity chromatography

Gene:
EGF

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone

Gene Symbols:
EGF

Primary or Secondary:
Primary

Content And Storage:
-20°C

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Antigen:
IL-17A

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Mouse

Gene Accession No.:
Q62386

Gene ID (Entrez):
__

Immunogen:
E.coli-derived Recombinant Murine IL-17A

Purification Method:
Antigen affinity chromatography

Gene:
IL17A

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A

Gene Symbols:
IL17A

Primary or Secondary:
Primary

Content And Storage:
-20°C

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7245539

Rabbit Polyclonal Antibody...


Antigen:
IL-17A

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Mouse

Gene Accession No.:
Q62386

Gene ID (Entrez):
__

Immunogen:
E.coli-derived Recombinant Murine IL-17A

Purification Method:
Antigen affinity chromatography

Gene:
IL17A

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A

Gene Symbols:
IL17A

Primary or Secondary:
Primary

Content And Storage:
-20°C

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Antigen:
CCL5 (RANTES)

Applications:
ELISA, Western Blot

Conjugate:
Biotin

Host Species:
Rabbit

Target Species:
Rat

Gene Accession No.:
P50231

Gene ID (Entrez):
81780

Immunogen:
E.coli-derived, 7.9 kDa Recombinant Rat RANTES (CCL5)

Purification Method:
Antigen affinity chromatography

Gene:
CCL5

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
beta-chemokine RANTES; CC chemokine; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; RANTES; RANTES protein; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; Scya5; SIS d; SISd; SIS-delta; SISd; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228; Unknown (protein for MGC:127014)

Gene Symbols:
CCL5

Primary or Secondary:
Primary

Content And Storage:
-20°C