CD134 (OX40) Monoclonal Antibody (OX-86), Super Bright™ 780, eBioscience™, Invitrogen™

Description

• Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F
• Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types
• The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding
• Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells
• Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells
• This 1RNM44N antibody will recognize a formaldehyde-fixed epitope
• Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• OX40 is a protein receptor found on the surface of T cells, a type of white blood cell that plays a crucial role in the immune system
• When activated, OX40 promotes the proliferation and survival of T cells, leading to a stronger immune response against cancer cells and infectious agents
• OX40 agonists, which are drugs that activate OX40, have shown promising results in preclinical and clinical studies as a potential immunotherapy for cancer
• They can enhance the efficacy of other cancer treatments such as chemotherapy and checkpoint inhibitors, and may also have applications in autoimmune diseases and allergies.