BASE-tag, Mouse anti-Virus, Clone: 7C7, Millipore Sigma™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AB131884100-Each-of-1 | In Stock | ₹ 41,308.37 |
AB131884100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,308.37
GST (18%): ₹ 7,435.507
Total Price: ₹ 48,743.877
Antigen
BASE-tag
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
Cap
Immunogen
Empty Adeno-associated virus 9 (AAV9) Capsids
Quantity
100 μL
Research Discipline
Inflammation & Immunology
Test Specificity
Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2b κ
Applications
Western Blot
Clone
7C7
Dilution
Immunoprecipitation: A representative lot immunoprecipitated EGFP-BASE-tag-Actin in HEK293T cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York). Immunofluorescence: A representative lot detected EGFP-BASE-tag-Actin in HeLa cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Gene Alias
Capsid protein VP1;Capsid protein AVV9
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Virus
Form
Purified
Related Products
Description
- Anti-BASE-tag, clone 7C7, Cat
- No
- MAB131884, is a mouse monoclonal antibody that recognizes the epitope QPAKKRLNFGQTGD and has been tested for use in Western Blotting, Immunoprecipitation, and Immunofluorescence
- Capsid protein VP1 (UniProt: Q6JC40) is encoded by the Cap gene in Adeno-associated virus 9 (AAV9)
- Adeno-associated viruses (AAV) are small non-enveloped DNA viruses that can serve as promising viral vectors for gene delivery, but can also elicits cellular and humoral immune responses
- AAVs are reported to be nonpathogenic in humans and animals and display low immunogenicity
- Following their attachment to cell surface receptor they are internalized via endocytosis and their intracellular trafficking is a rate-limiting, slow and inefficient process
- AAVs contain an icosahedral capsid, which is 22 to 26 nm in diameter
- They are shown to have a single-stranded DNA genome of approximately 4.7 kilobases that is flanked by two T-shaped inverted terminal repeats (ITRs)
- The 7C7 an epitope-tag antibody recognizes a basic ten amino acid epitope (QPAKKRLNFGQTGD) with high specificity
- The basic nature of the BASE-tag epitope sequence distinguishes it from other commonly used epitope tags, such as Myc, EQKLISEEDL, FLAG, DYKDDDDK, HA, YPYDVPDYA, V5 etc
- Clone 7C7 recognizes capsid proteins VP1 and VP2, but not VP3
- Based on sequence identity of the epitope sequence, this antibody is expected to recognize VP1 and VP2 of AAV serotypes 1,2,3,6,7,8, and 9
- (Ref.: Nonnenmacher, M., and Weber, T (2012)
- Gene Ther
- 19(6); 649-658).
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Antigen: BASE-tag
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Cap
Immunogen: Empty Adeno-associated virus 9 (AAV9) Capsids
Quantity: 100 μL
Research Discipline: Inflammation & Immunology
Test Specificity: Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b κ
Applications: Western Blot
Clone: 7C7
Dilution: Immunoprecipitation: A representative lot immunoprecipitated EGFP-BASE-tag-Actin in HEK293T cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York). Immunofluorescence: A representative lot detected EGFP-BASE-tag-Actin in HeLa cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Gene Alias: Capsid protein VP1;Capsid protein AVV9
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Virus
Form: Purified
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