Leptin Monoclonal Antibody (11), Invitrogen™

Description

• ABS 022-11-02 detects Leptin in human samples
• ABS 022-11-02 has been successfully used in ELISA and Western blot procedures
• ABS 022-11-02 binds free Leptin in solution
• In sandwich ELISA, ABS 022-11-02 works as a detection antibody with ABS 022-03-02 as a capture antibody
• ABS 022-11-02 shows three bands at 16, 32, and 48 kDa in the Western blot application corresponding with the leptin monomer, dimer and trimer forms
• NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function
• Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene
• Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body
• Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta
• Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells
• Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure
• Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors
• Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth
• Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension
• Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation
• Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.