ABE194725UG
PRDM9, Rabbit anti-Mouse, Human, Polyclonal, Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE194725UG-Each-of-1 | In Stock | ₹ 16,456.61 |
ABE194725UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
PRDM9
Classification
Polyclonal
Dilution
Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate ( Courtesy of Guccione Lab @ A*STAR( Institute of Molecular Cell Biology, IMCB) ).
Gene Alias
Histone-lysine N-methyltransferase PRDM9;EC: 2.1.1.43;PR domain zinc finger protein 9;PR domain-containing protein 9
Host Species
Rabbit
Molecular Weight of Antigen
∽90 kda observed; 103.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity
25 μg
Research Discipline
Epigenetics & Nuclear Function
Test Specificity
This rabbit polyclonal antibody detects Histone-lysine N-methyltransferase PRDM9 in human and mouse cells. It targets an epitope within 191 amino acids from the N-terminal half.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG
Applications
Western Blot
Conjugate
Unconjugated
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
PRDM9;PFM6
Immunogen
GST-tagged recombinant fragment corresponding to 191 amino acids from the N-terminal half of human Histone-lysine N-methyltransferase PRDM9.
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Mouse, Human
Form
Purified
Description
- Anti-PRDM9, Cat
- No
- ABE1947, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase PRDM9 in human and murine cells and has been tested for use in Western Blotting
- Histone-lysine N-methyltransferase PRDM9 (UniProt: Q9NQV7; EC: 2.1.1.43, PR domain zinc finger protein 9, PR domain-containing protein 9, PRDM9) is encoded by PRDM9 (also known as PFM6) gene (Gene ID: 56979) in human
- PRDM9 is a zinc-finger protein that catalyzes histone H3 Lysine 4 trimethylation (H3K4me3), but lacks capacity to mono- and dimethylate Lysine 4 of histone H3
- H3 Lysine -4 methylation plays a vital role in the transcriptional activation of genes during early meiotic prophase
- PRDM9 contains multiple domains, including a Kruppel-associated box (KRAB) domain (aa 23-86), an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain (aa 244-358), and a tandem array of C2H2 zinc fingers
- The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity
- PRDM9 is reported to be highly polymorphic, both within and between mammalian species
- It is shown that most of the naturally occurring sequence polymorphisms in PRDM9 change the identity of the amino acids contacting DNA and/or the number and arrangement of individual fingers in the DNA-binding zinc-finger domains
- This allows PRDM9 variants to target a large number of DNA sequences, thereby expanding the distribution of recombination sites
- (Ref.: Baker, CL et al
- (2015)
- PLoS Genet 11(9): e1005512).
Compare Similar Items
Show Difference
Antigen: PRDM9
Classification: Polyclonal
Dilution: Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate ( Courtesy of Guccione Lab @ A*STAR( Institute of Molecular Cell Biology, IMCB) ).
Gene Alias: Histone-lysine N-methyltransferase PRDM9;EC: 2.1.1.43;PR domain zinc finger protein 9;PR domain-containing protein 9
Host Species: Rabbit
Molecular Weight of Antigen: ∽90 kda observed; 103.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity: 25 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: This rabbit polyclonal antibody detects Histone-lysine N-methyltransferase PRDM9 in human and mouse cells. It targets an epitope within 191 amino acids from the N-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG
Applications: Western Blot
Conjugate: Unconjugated
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: PRDM9;PFM6
Immunogen: GST-tagged recombinant fragment corresponding to 191 amino acids from the N-terminal half of human Histone-lysine N-methyltransferase PRDM9.
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Mouse, Human
Form: Purified
Antigen:
PRDM9
Classification:
Polyclonal
Dilution:
Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate ( Courtesy of Guccione Lab @ A*STAR( Institute of Molecular Cell Biology, IMCB) ).
Gene Alias:
Histone-lysine N-methyltransferase PRDM9;EC: 2.1.1.43;PR domain zinc finger protein 9;PR domain-containing protein 9
Host Species:
Rabbit
Molecular Weight of Antigen:
∽90 kda observed; 103.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity:
25 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
This rabbit polyclonal antibody detects Histone-lysine N-methyltransferase PRDM9 in human and mouse cells. It targets an epitope within 191 amino acids from the N-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG
Applications:
Western Blot
Conjugate:
Unconjugated
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
PRDM9;PFM6
Immunogen:
GST-tagged recombinant fragment corresponding to 191 amino acids from the N-terminal half of human Histone-lysine N-methyltransferase PRDM9.
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Mouse, Human
Form:
Purified
Antigen: RLIM/Rnf12
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μL
Research Discipline: Epigenetics and Nuclear Function
Test Specificity: __
Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype: __
Applications: ChIP Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Conjugate: __
Formulation: Rabbit polyclonal serum with 0.05% sodium azide.
Gene Symbols: Rlim; Rnf12
Immunogen: Recombinant protein corresponding to mouse RLIM/Rnf12.
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Hamster, Human, Mouse
Form: Serum
Antigen:
RLIM/Rnf12
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μL
Research Discipline:
Epigenetics and Nuclear Function
Test Specificity:
__
Content And Storage:
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype:
__
Applications:
ChIP Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Conjugate:
__
Formulation:
Rabbit polyclonal serum with 0.05% sodium azide.
Gene Symbols:
Rlim; Rnf12
Immunogen:
Recombinant protein corresponding to mouse RLIM/Rnf12.
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Hamster, Human, Mouse
Form:
Serum
Antigen: RAD9A
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: This rabbit polyclonal antibody detected nuclear RAD9A immunoreactivity in human and mouse tissue sections.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt..
Isotype: __
Applications: Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: GST-tagged recombinant full-length mouse RAD9A.
Purification Method: Protein A Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
RAD9A
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
This rabbit polyclonal antibody detected nuclear RAD9A immunoreactivity in human and mouse tissue sections.
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt..
Isotype:
__
Applications:
Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
GST-tagged recombinant full-length mouse RAD9A.
Purification Method:
Protein A Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: Borealin
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 50 μL
Research Discipline: Epigenetics and Nuclear Function
Test Specificity: This polyclonal antibody detects Borealin in nocodozole treated HeLa cells.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: __
Applications: Western Blot, Immunofluorescence, Immunoprecipitation
Conjugate: Unconjugated
Formulation: __
Gene Symbols: CDCA8,PESCRG3
Immunogen: A His-tagged full length human recombinant Borealin.
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Serum
Antigen:
Borealin
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
50 μL
Research Discipline:
Epigenetics and Nuclear Function
Test Specificity:
This polyclonal antibody detects Borealin in nocodozole treated HeLa cells.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
__
Applications:
Western Blot, Immunofluorescence, Immunoprecipitation
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
CDCA8,PESCRG3
Immunogen:
A His-tagged full length human recombinant Borealin.
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Serum