AMPA6504

HIF1A Monoclonal Antibody (H1alpha67), Invitrogen™

Manufacturer: Thermo Scientific

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Pack Size SKU Availability Price
Each of 1 AMPA6504-Each-of-1 In Stock ₹ 50,352.06

AMPA6504 - Each of 1

₹ 50,352.06

In Stock

Quantity

1

Base Price: ₹ 50,352.06

GST (18%): ₹ 9,063.371

Total Price: ₹ 59,415.431

Antigen

HIF1A

Classification

Monoclonal

Concentration

1.0 mg/mL

Formulation

PBS with 1% BSA and 0.05% sodium azide

Gene Accession No.

O35800, Q16665, Q61221, Q9I8A9, Q9XTA5

Gene Symbols

hif-1a, HIF1A, hif1a.L

Immunogen

Fusion protein containing residues 432-528 of human HIF-1alpha.

Quantity

100 μL

Primary or Secondary

Primary

Target Species

Bovine, Canine, Feline, Ferret, Human, Mouse, Non-human Primate, Ovine, Porcine, Rabbit, Rat, X. laevis

Product Type

Antibody

Isotype

IgG2b

Applications

ChIP Assay, ELISA, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, Proximity Ligation Assay, Western Blot

Clone

H1alpha67

Conjugate

Unconjugated

Gene

HIF1A

Gene Alias

AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

Host Species

Mouse

Purification Method

Protein G

Regulatory Status

RUO

Gene ID (Entrez)

100009579, 100337573, 101083686, 15251, 281814, 29560, 3091, 380141, 396696, 443519, 445838, 480348

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form

Liquid

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Description

  • In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha
  • For WB, testing on nuclear extracts is recommended
  • Recommended positive controls: COS-7 nuclear hypoxic induced cell lysate, HeLa hypoxic (CoCl2) cell lysate, HepG2 hypoxic (CoCl2) cell lysate, HeLa hypoxic cell lysate, HepG2 hypoxic cell lysate, HIF-1 alpha Knockout HeLa cell lysate HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension
  • HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors
  • HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia
  • Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia
  • HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis
  • Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions
  • Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation
  • In humans, it is located on the q arm of chromosome 14
  • The C-terminal of HIF1A binds to p300
  • p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF
  • Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Compare Similar Items

Show Difference

Img

Thermo Scientific

AMPA6504

--


Antigen:
HIF1A

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Formulation:
PBS with 1% BSA and 0.05% sodium azide

Gene Accession No.:
O35800, Q16665, Q61221, Q9I8A9, Q9XTA5

Gene Symbols:
hif-1a, HIF1A, hif1a.L

Immunogen:
Fusion protein containing residues 432-528 of human HIF-1alpha.

Quantity:
100 μL

Primary or Secondary:
Primary

Target Species:
Bovine, Canine, Feline, Ferret, Human, Mouse, Non-human Primate, Ovine, Porcine, Rabbit, Rat, X. laevis

Product Type:
Antibody

Isotype:
IgG2b

Applications:
ChIP Assay, ELISA, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, Proximity Ligation Assay, Western Blot

Clone:
H1alpha67

Conjugate:
Unconjugated

Gene:
HIF1A

Gene Alias:
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

Host Species:
Mouse

Purification Method:
Protein G

Regulatory Status:
RUO

Gene ID (Entrez):
100009579, 100337573, 101083686, 15251, 281814, 29560, 3091, 380141, 396696, 443519, 445838, 480348

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form:
Liquid

Img

Thermo Scientific

AMPA6511

--


Antigen:
HIF1A

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Formulation:
PBS with 1% BSA and 0.05% sodium azide

Gene Accession No.:
O35800, Q16665, Q61221, Q9XTA5

Gene Symbols:
HIF1A

Immunogen:
Fusion protein containing residues 432-528 of human HIF-1alpha

Quantity:
100 μL

Primary or Secondary:
Primary

Target Species:
Avian, Bovine, Canine, Ferret, Human, Mouse, Non-human Primate, Ovine, Porcine, Rabbit, Rat

Product Type:
Antibody

Isotype:
IgG2b

Applications:
ChIP Assay, ELISA, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot

Clone:
H1alpha67

Conjugate:
Unconjugated

Gene:
HIF1A

Gene Alias:
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

Host Species:
Mouse

Purification Method:
Protein A

Regulatory Status:
RUO

Gene ID (Entrez):
100009579, 15251, 281814, 29560, 3091, 396696, 443519, 480348

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form:
Liquid

Img

Thermo Scientific

AMPA6512

--


Antigen:
HIF-1 beta

Classification:
Monoclonal

Concentration:
1 mg/mL

Formulation:
PBS with 0.05% sodium azide

Gene Accession No.:
P27540, P41739, P53762, Q9BE97

Gene Symbols:
ARNT

Immunogen:
Fusion protein containing residues 496-789 of human ARNT.

Quantity:
100 μL

Primary or Secondary:
Primary

Target Species:
Bovine, Ferret, Human, Mouse, Non-human Primate, Ovine, Rat

Product Type:
Antibody

Isotype:
IgG1 κ

Applications:
ChIP Assay, ChIP sequencing (ChIP-seq), Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot

Clone:
H1beta234

Conjugate:
Unconjugated

Gene:
ARNT

Gene Alias:
AHA-1; ARNT; ARNT protein; Arnt1; ARNT1a; ARNT1b; aryl hydrocarbon receptor nuclear translocater; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; aryl hydrocarbon receptor nuclear translocator type 1a; aryl hydrocarbon receptor nuclear translocator type 1b; bHLHe2; Class E basic helix-loop-helix protein 2; D3Ertd557e; dioxin receptor, nuclear translocator; Drnt; ESTM42; hif 1; HIF1 beta; HIF-1 beta; Hif1b; HIF1BETA; HIF-1beta; HIF-1-beta; HIF1-beta; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; hypoxia-inducible factor 1-beta; mKIAA4051; TANGO; W08714; zgc:136664

Host Species:
Mouse

Purification Method:
Protein G

Regulatory Status:
RUO

Gene ID (Entrez):
11863, 25242, 281010, 405, 443285

Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles

Form:
Liquid

Img

Thermo Scientific

AMPA6517

--


Antigen:
HIF1A

Classification:
Monoclonal

Concentration:
1.5 mg/mL

Formulation:
PBS with 0.05% sodium azide

Gene Accession No.:
Q16665

Gene Symbols:
HIF1A

Immunogen:
Fusion protein containing residues 432-528 of human HIF-1 alpha.

Quantity:
100 μL

Primary or Secondary:
Primary

Target Species:
Human, Non-human Primate

Product Type:
Antibody

Isotype:
IgG2b

Applications:
Western Blot

Clone:
H1alpha 67-7

Conjugate:
Unconjugated

Gene:
HIF1A

Gene Alias:
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L home

Host Species:
Mouse

Purification Method:
Protein G

Regulatory Status:
RUO

Gene ID (Entrez):
3091

Content And Storage:
4° C, do not freeze

Form:
Liquid