CD8 Mouse anti-Human, FITC, Clone: SK1, BD

Description

• CD8 recognizes the 32-kilodalton (kd) α-subunit of a disulfide-linked bimolecular complex.The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (Mr 32, 30 kd), while CD8+ CD16+ natural killer (NK) lymphocytes and CD8+ T-cell receptor (TCR)-γ/δ + T lymphocytes express an α/α homodimer (Mr 30 kd)
• CD8+ TCR-α/β+ T lymphocytes can express either an α/α homodimer or α/β heterodimer
• The CD8 antigenic determinant binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells.Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes.The CD8 antigen is coupled to a protein tyrosine kinase, p56 lck
• The CD8:p56 lck complex can play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3/TCR complex
• The CD8 antigen is present on the human suppressor/cytotoxic T-lymphocyte subset as well as on a subset of NK lymphocytes.The CD8 antigen is expressed on 19% to 48% of normal peripheral blood lymphocytes and 60% to 85% of normal thymocytes.The CD8+ T- and NK-lymphocyte subsets can be further subdivided into the following groups: CD16+ NK lymphocytes that can express the CD8 antigen in low density;CD57+ T lymphocytes that express high-density CD8 antigen;and CD8+CD62L+ lymphocytes that collaborate with CD8+ CD62Ln++hocytes to generate suppression of B-lymphocyte function.CD8 cross-reacts with lymphocytes of some nonhuman primate species Flow Cytometry