PIR-A/B Rat anti-Mouse, Unlabeled, Clone: 6C1, BD

Description

• The 6C1 antibody reacts with common epitopes of PIR-A and PIR-B (Paired Immunoglobulin-liked Receptors) in all mouse strains tested (A/J, BALB/cJ, C3H, C57BL/6, DBA/1, DBA/2, NZB, and SJL)
• PIR-A and PIR-B are type-I transmembrane glycoproteins containing six Ig-like domains
• There are multiple PIR-A proteins which are activating receptors by virtue of their intracellular association with the ITAM (Immunoreceptor Tyrosine-based Activation Motif)- containing Fc receptor γ chain (FcRγ) in mast cells and macrophages
• FcRγ expression is required for PIR-A cell-surface expression on dendritic cells, B cells, and myeloid lineages
• In contrast, PIR-B is an inhibitory receptor which contains various ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs) in its cytoplasmic domain
• These receptors are expressed on B cells, granulocytes, mast cells, dendritic cells, and monocytes/macrophages, but not on thymocytes, T lymphocytes, erythroid lineage cells, or NK cells
• The level of cell-surface expression of these receptors increases as a function of B-cell activation and myeloid- and B-lineage differentiation
• The 6C1 antibody immunoprecipitates molecules of 85- and 125kDa, which correspond to PIRA and PIR-B, respectively
• Host Species: Rat Clone: 6C1 Isotype: IgG1 κ Species Reactivity: Mouse Immunogen: Recombinant mouse PIR-A1 extracellular domain peptide and the mouse myeloid cell line, WEH13 Flow Cytometry, Immunoprecipitation