PARP (Asp214), Cleaved Mouse anti-Human, PE, Clone: F21-852, BD

Description

• PARP (Poly [ADP-Ribose] Polymerase) is a 113-kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself
• The catalytic activity of PARP is increased in cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage
• Additionally, PARP is a target of the caspase protease activity associated with apoptosis
• The PARP protein consists of an N-terminal DNA-binding domain (DBD) and a C-terminal catalytic domain separated by a central automodification domain
• During apoptosis, Caspase-3 cleaves PARP at a recognition site (Asp Glu Val Asp Gly) in the DBD to form 24- and 89-kDa fragments
• This process separates the DBD (which is mostly in the 24-kDa fragment) from the catalytic domain (in the 89-kDa fragment) of the enzyme, resulting in the loss of normal PARP function
• It has been proposed that inactivation of PARP directs DNA-damaged cells to undergo apoptosis rather than necrotic degradation, and the presence of the 89-kDa PARP cleavage fraction is considered to be a marker of apoptosis
• A peptide corresponding to the N-terminus of the cleavage site (Asp 214) of human PARP was used as the immunogen
• The F21-852 monoclonal antibody reacts only with the 89-kDa fragment of human PARP-1 that is downstream of the Caspase-3 cleavage site (Asp214) and contains the automodification and catalytic domains
• It does not react with intact human PARP-1
• Cross-reactivity with other members of the PARP superfamily is unknown
• It may also recognize cleaved PARP in a number of other species due to the conserved nature of the molecule, although this has not been tested at BD Biosciences Pharmingen
• Host Species: Mouse Clone: F21-852 Isotype: IgG1 κ Species Reactivity: Human Immunogen: Human cleaved PARP Flow Cytometry, Immunoprecipitation, Intracellular Staining, Western Blotting