CD24 Rat anti-Mouse, Unlabeled, Clone: J11D, BD

Description

• The J11d antibody reacts with CD24 (Heat Stable Antigen, HSA or HsAg), a variably glycosylated membrane protein found on erythrocytes, granulocytes, monocytes, and lymphocytes
• Levels of expression of CD24 vary during differentiation of the T and B cell lineages: Immature B cells in the bone marrow and spleen of adult mice express high levels of CD24, whereas mature peripheral B cells express intermediate levels of CD24
• Similarly, the majority of thymocytes express high levels of CD24, while mature thymic and peripheral T cells do not express CD24
• Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24
• CD24 is not expressed by NK cells
• CD24 is a ligand of CD62P (P-selectin)
• In vitro treatment of splenocytes with J11d antibody plus complement reduces primary IgM responses and proliferative responses to LPS
• While the monoclonal antibodies 30-F1 (Cat
• no
• 558777), M1/69 (Cat
• no
• 557436), and J11d all react with CD24, they show subtle differences in the level of staining of different cell populations
• When possible, investigators should continue to use the same monoclonal antibody as used in previous studies.