CD44 Rat anti-Mouse, NA/LE, Unlabeled, Clone: KM114, BD

Description

• The KM114 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24)
• The KM114 hybridoma was produced at the same time as the published clone KM201, and the mAb recognizes a different epitope from that recognized by mAb IM7
• The standard form of CD44, lacking variable exons and referred to as CD44H or CD44S, is widely expressed on hematopoietic and non-hematopoietic cells
• CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes
• Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have lower expression of CD44H on T cells
• CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices
• Differential glycosylation of CD44 influences its binding to hyaluronate
• Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1).10 Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44
• In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells; and memory cells can be recognized by their CD44[hi] phenotype
• KM114 antibody can be used in ELISA to detect soluble CD44, and it is effective for in vitro blocking of hyaluronate recognition by CD44
• It has been reported that KM114 mAb cross-reacts with CD44 of the Chinese hamster, Cricetulus griseus , but not human CD44.