CD1d Rat anti-Mouse, Biotin, Clone: 1B1, BD

Description

• The 1B1 monoclonal antibody specifically binds to mouse CD1d, a 48-kDa glycoprotein with structural homology to the major histocompatibility complex (MHC) class I molecules
• The structure, expression, and functions of CD1 antigens are complex and have been reviewed
• mAb 1B1 detects CD1d at varying levels on most types of bone marrow and peripheral leukocytes and on epithelial, dendritic, and lymphoid cells in the thymus
• It appears to recognize CD1d only in association with β2m
• CD1d has been reported to be expressed by gastrointestinal tract epithelium and in the cytoplasm of hepatocytes as detected via immunohistochemical staining of frozen sections with mAb 3C11 (Cat
• No
• 559871, for the purified antibody), suggesting a possible role for CD1d in mucosal immunity
• However, CD1d expression was not detectable, via flow cytometry, on intestinal epithelial cells in studies using the anti-CD1d mAbs 3C11, 1B1, and 9C7
• The 1B1 antibody competes with mAb 3C11 in binding to mouse splenocytes
• This antibody is routinely tested by flow cytometric analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.