Cyclin B1 Mouse, FITC, Clone: GNS-1, BD

Description

• Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes
• Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle
• The main cyclin-cdks complexes formed in vertebrae cells are cyclin-cdks complexes that regulate the progression of the cell cycle
• The main cyclin-cdks complexes formed in vertebrae cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M)
• These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27[Kip1]
• Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb) nucleolin and others
• Cyclin B1 is a mitotic cyclin complex; expression is normally low in G0/G1, increases in S, and is maximal during G2/M
• Cyclin B1 is rapidly degraded at the end of mitosis and is required for cells to exit from mitosis
• GNS-1 recognizes an epitope between amino acids 1-21 of human Cyclin B1
• It crossreacts with hamster and mouse cyclin B1
• Recombinant human Cyclin B1 was used as immunogen
• The antibody was originally characterized by western blot analysis, immunoprecipitation, immunohistochemical staining of acetone-fixed, frozen tissue sections and of formalin-fixed, paraffin-embedded tissue sections, indirect immunofluorescence microscopy of cultured cells and flow cytometry
• MOPC-1 is used as a mouse IgG1 isotype (negative) control
• The MOPC-21 antibody has unknown specificity
• The GNS-1 and MOPC-21 FITC conjugates are matched in F/P ratios
• The optimum F/P ratio was experimentally determined by flow cytometric analysis
• MOLT4 human leukemia cells (ATCC CRL-1582) are recommended as a positive control for this application
• Host Species: Mouse Clone: GNS-1, MOPC-21 Isotype: IgG1, IgG1 κ Species Reactivity: Human Immunogen: Human Cyclin B1 Recombinant Protein Flow Cytometry