p53 Mouse, Unlabeled, Clone: PAB 240, BD

Description

• p53 is a nuclear phosphoprotein which acts as a tumor suppressor by providing a cell cycle checkpoint for DNA damage during S-phase
• Mutations in wildtype p53 can indirectly alter the DNA binding and transcription factor activity of p53
• By altering expression of genes normally regulated by p53, these mutations can result in both a loss of tumor suppressor function and a gain of oncogenic function
• The majority of mutations in the p53 gene are missense mutations which alter the identity of an amino acid
• These mutations may alter the conformation and thus increase the stability of the mutant p53 protein
• p53 is expressed in all vertebrate species examined
• p53 may be overexpressed in transformed cell lines, where it forms complexes with viral oncogenes including SV40 large T antigen and the adenovirus protein, E1B
• p53 migrates at ∽53kDa on SDS-PAGE
• PAb 240 reacts with a conformational epitope between amino acids 156 and 214 of native p53
• As such, Pab 240 recognizes only certain mutant forms of p53, as determined by immunoprecipitation
• It detects both mutant and wildtype p53 in western blot analysis and immunohistochemistry of frozen tissue sections
• It is thought that p53 mutations exert a common conformational change which results in expression of the Pab 240-specific epitope on mutant p53 molecules
• A recombinant fusion protein of p53 sequence including amino acids 14-289 was used as immunogen
• Host Species: Mouse Clone: PAb 240 Isotype: IgG1 Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine, Chicken Immunogen: Human p53 aa
• 14-289 Formula Weight [Chemical]: 53kDa Immunohistochemistry (Frozen), Immunoprecipitation, Western Blotting