CD95 Hamster anti-Mouse, FITC, Clone: JO2, BD

Description

• Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis
• It belongs to the TNF (tumor necrosis factor)/NGF receptor family
• Expression of Fas has been described in the thymus, liver, heart, lung and ovary
• Fas plays an important role in the apoptotic process that takes place during development
• Monoclonal antibodies recognizing Fas such asJo2 have cytolytic activity on cells expressing Fas
• The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis
• The Jo2 antibody recognizes mouse Fas
• The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis
• Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death
• Jo2 mAb has been reported to immunoprecipitate mouse Fas as a 45 kDa band from W4 cells
• W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas
• The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation.