CD178 Mouse anti-Human, NA/LE, Unlabeled, Clone: NOK-2, BD

Description

• Fas (APO-1, CD95) is a 45kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL)
• Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary, and lung
• FasL is a 40kDa TNF family membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor
• FasL is expressed on activated T and NK cells
• Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development
• NOK-2 recognizes human FasL
• It recognizes both the membrane bound (FasL) and soluble (sFasL) forms
• L5178Y mouse T lymphoma cells expressing recombinant human FasL were used as immunogen
• FasL and sFasL migrate at reduced molecular weights of 40 and 26kDa, respectively
• However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al.